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AB325631

Human TrkB ELISA Kit, Chemiluminescent

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Human TrkB ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TrkB with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 9.6 - 21000 pg/ml with a sensitivity of 3.02 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending
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Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)
  • sELISA

Lab

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)
  • sELISA

Lab

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)

Standard curve comparison between the Chemiluminescent Human TrkB SimpleStep ELISA and colorimetric Human TrkB SimpleStep ELISA (ab264625) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)
  • sELISA

Lab

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)

Dynamic range comparison between the Chemiluminescent Human TrkB SimpleStep ELISA and colorimetric Human TrkB SimpleStep ELISA (ab264625). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)
  • sELISA

Lab

Sandwich ELISA - Human TrkB ELISA Kit, Chemiluminescent (AB325631)

Interpolated concentrations of native TrkB in human brain extract sample based on a 1,000 ug/mL extract load. The concentrations of TrkB were measured in duplicate and interpolated from the TrkB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TrkB concentration was determined to be 1,934 pg/mL in brain extract.

주요 정보

검출 방식

Luminescent

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.02 pg/mL

검출 범위

9.6 - 21000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Primary Antibodies

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Anti-Pan Trk antibody [EPR17341]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (AB181560)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human TrkB ELISA Kit, Chemiluminescent (ab325631) is a rapid single-wash sandwich 55 min ELISA to measure Human TrkB in Tissue Extract with a sensitivity of 3.02 pg/mL.

How the assay works

Human TrkB ELISA Kit, Chemiluminescent (ab325631) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TrkB (ab325631) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TrkB antibody [EPR18925-15] - BSA and Azide free (Capture) ab267457
- Anti-TrkB antibody [EPR18925-48] - BSA and Azide free (Detector) ab242343

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.6" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "108 - 118 %", "average": "= 112" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325631-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human TrkB Detector Antibody", "number":"AB325631-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB325631-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325631-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TrkB Capture Antibody", "number":"AB325631-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TrkB Lyophilized Recombinant Protein", "number":"AB325631-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325631-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325631-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325631-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325631-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325631-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325631-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
See full target information NTRK2

대체 명칭 보기

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B
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websiteProtocolBooklet
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Product promise

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