JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB192149

Human TSLP ELISA Kit

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Human TSLP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TSLP with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
5 이미지
ELISA - Human TSLP ELISA Kit (AB192149)
  • ELISA

Supplier Data

ELISA - Human TSLP ELISA Kit (AB192149)
Sandwich ELISA - Human TSLP ELISA Kit (AB192149)
  • sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Titration of normal Human serum (NHS) native and spiked with 0.5 ng/mL recombinant TSLP protein to within the working range of the assay.

The samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)
  • sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Assay for TSLP levels from 10 Individual Donor Human Serum samples and patients clinically diagnosed with Atopic Dermatitis (A.D.) to within the working range of the assay.

Severity of A.D. symptoms and medical histories of all donors are unknown. Samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)
  • sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Example of TSLP standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)
  • sELISA

Supplier Data

Sandwich ELISA - Human TSLP ELISA Kit (AB192149)

Example of TSLP standard curve prepared in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3 pg/mL

검출 범위

15.6 - 1000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Microplate

style
left-column

보완 제품

Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0
Assay Kits

AB272200

Human IL-25 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-25 ELISA Kit (AB272200)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB214030

Human IL-8 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

  • 5
  • 2
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human TSLP ELISA Kit ab192149 is a rapid single-wash 90-min Sandwich ELISA to measure Human TSLP in cell culture supernatant, serum. This SimpleStep sensitivity is 3 pg/mL.

How the assay works

Human TSLP SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TSLP ELISA Kit ab192149 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Thymic stromal lyphopoietin (TSLP) is a protein belonging to the IL-7 cytokine family. TSLP is released by epithelial cells and keratinocytes in response to trauma, infection or allergen. It acts upon dendritic cells to mediate TH2 mediated immune response and appears to be a key mediator in many inflammatory diseases such as: Asthma, Eczema, and Atopic Dermatitis. TSLP also acts upon mast cells promote the release of IL-5, -6, and -13 in the presence of IL-1 and TNF-?.

style
left-column

Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

Recovery

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "114 - 126 %", "average": "= 121" } ]

style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab192149-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB192149-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB192149-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB192149-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB192149-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB192149-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB192149-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB192149-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB192149-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TSLP Detector Antibody", "number":"AB192149-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TSLP Lyophilized Recombinant Protein", "number":"AB192149-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TSLP Capture Antibody", "number":"AB192149-CMP09", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7R?).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7R? receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.
See full target information TSLP

대체 명칭 보기

Thymic stromal lymphopoietin, TSLP
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of thoracic disease 10:6585-6597 PubMed30746204

2019

Excessive periostin expression and Th2 response in patients with nasal polyps: association with asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wei,Renqiang Ma,Jia Zhang,Xingmei Wu,Guodong Yu,Xianting Hu,Jian Li,Zhuofu Liu,Wendong Ji,Huabin Li,Weiping Wen
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com