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AB289701

Human Vasopressin-neurophysin 2-copeptin ELISA Kit

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Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vasopressin-neurophysin 2-copeptin with a sensitivity of 12.034 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
2 이미지
Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

Example of human Vasopressin Neurophysin 2-copeptin standard curve in 1X Cell Extraction Buffer PTR.

Example of human Vasopressin Neurophysin 2-copeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

Interpolated concentrations of human Vasopressin Neurophysin 2-copeptin in human brain stem extract.

Interpolated concentration of native Vasopressin Neurophysin 2-copeptin was measured in duplicate at different sample concentrations. Undiluted samples are 600 μg/mL human brain stem extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 12.034 pg/mL

검출 범위

40.625 - 2600 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vasopressin-neurophysin 2-copeptin with 12.034 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

Recovery

[ { "sample": "Tissue Extracts", "range": "94 - 96 %", "average": "= 95" } ]

제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

Arg-vasopressin. Has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2).. Neurophysin 2. Specifically binds vasopressin.
See full target information AVP

대체 명칭 보기

ARVP, VP, AVP, Vasopressin-neurophysin 2-copeptin, AVP-NPII

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of translational research 14:8002-8008 PubMed36505320

2022

The value of Copeptin, myocardial fatty acid-binding protein and myocardial markers in the early diagnosis of acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Guan,Tian Liang,Jianyun Fan,Wenyou Yang,Dongliang Wu,Xinguo Li
제품이 사용된 논문 모두 보기
websiteProtocolBooklet
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