Human VEGF ELISA Kit, Chemiluminescent

• sELISA

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Serial dilutions of recombinant human VEGF189 and VEGF121 were prepared and assayed in parallel with recombinant VEGF165.

• sELISA

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Dynamic range comparison between the Chemiluminescent Human VEGF SimpleStep ELISA, colorimetric Human VEGF SimpleStep ELISA (ab222510), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

• sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Standard curve comparison between the Chemiluminescent Human VEGF SimpleStep ELISA and colorimetric Human VEGF SimpleStep ELISA (ab222510) in Sample Diluent NS +1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Comparison of VEGF in PHA-M stimulated and unstimulated human PBMC cell culture supernatants stimulated for different durations.

Human PBMC (seeded at 10x10⁶/mL) were cultured for 2 or 5 days in the presence or absence of 1.5% PHA-M. The concentrations of VEGF were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the VEGF standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean VEGF concentration was determined to be 34.76 pg/mL in 2 Day PHA-M stimulated PBMC cell culture supernatant, 393.7 pg/mL in 5 Day PHA-M stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant at both 2 and 5 days (not shown).

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 235.0 pg/mL with a range of 81.10 – 993.3 pg/mL.

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Interpolated concentrations of native VEGF in human breast milk (de-fatted), saliva, and urine samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : breast milk 2%, saliva 12.5%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 21487 pg/mL in neat human breast milk (de-fatted), 3712 pg/mL in neat human saliva, and 262.0 pg/mL in neat human urine.

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Interpolated concentrations of native VEGF in human serum and plasma samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 268.9 pg/mL in neat serum, 123.9 pg/mL in neat plasma (citrate), 133.8 pg/mL in neat plasma (heparin), and 95.76 pg/mL in neat plasma (EDTA).

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Interpolated concentrations of native VEGF in PC-3 cell extract (1 Day), PC-3 cell extract (2 Day), HepG2 cell extract, A549 cell extract, and MDA-MB-435S cell extract samples based on a 300 μg/mL extract load. The concentrations of VEGF were measured in duplicate and interpolated from the VEGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 86.65 pg/mL in PC-3 cell extract (1 Day), 149.6 pg/mL in PC-3 cell extract (2 Day), 127.7 pg/mL in HepG2 cell extract, 241.7 pg/mL in A549 cell extract, and 454.5 pg/mL in MDA-MB-435S cell extract.

• sELISA

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Interpolated concentrations of native VEGF in cell culture supernatant samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 5%, A431 5%, MDA-MB-435S 12.5%, PC-3 20%, A549 50%, and PBMC 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 5027 pg/mL in neat HepG2 supernatant, 7356 pg/mL in neat A431 supernatant (4 Day), 5918 pg/mL in neat MDA-MB-435S supernatant, 1495 pg/mL in neat PC-3 (2 Day), 603.4 pg/mL in neat A549 supernatant, and 405.3 pg/mL in neat PBMC supernatant (PHA-M, 5 Day).

• sELISA

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Sandwich ELISA - Human VEGF ELISA Kit, Chemiluminescent (AB326899)

Other species reactivity was determined by measuring a 500 pg/mL protein load of various species recombinant VEGF proteins.

Protein concentrations were interpolated from the human standard curve, and graphing the interpolated concentrations (mean +/- SD, n=2).