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AB246526

Human Vimentin ELISA Kit

Human Vimentin ELISA 키트

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(2 제품이 사용된 논문 )

Human Vimentin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vimentin with a sensitivity of 1.34 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 이미지
Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Example of human Vimentin standard curve in 1X Cell Extraction Buffer PTR.

The Vimentin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Interpolated concentrations of native Vimentin in human cell extracts at a 250 ng/mL extract load.

The concentrations of Vimentin were measured in duplicate and interpolated from the Vimentin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vimentin concentration was determined to be 65.2 ng/mL in A549 extracts.

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Representative image of Human Vimentin ELISA Kit (ab246526)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Vimentin Capture Antibody

- 10X Human Vimentin Detector Antibody

- Human Vimentin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.34 ng/mL

검출 범위

1.5625 - 100 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Vimentin ELISA Kit (ab246526) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vimentin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vimentin with 1.34 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "88 - 103 %", "average": "= 99" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246526-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246526-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246526-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246526-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246526-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB246526-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246526-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246526-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246526-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB246526-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Vimentin Lyophilized Recombinant Protein", "number":"AB246526-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vimentin Capture Antibody", "number":"AB246526-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vimentin Detector Antibody", "number":"AB246526-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM

대체 명칭 보기

Vimentin, VIM
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Mucosal immunology 17:124-136 PubMed38007005

2023

Salivary IgA and vimentin differentiate in vitro SARS-CoV-2 infection: A study of 290 convalescent COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Ellis,Rosie Way,Miranda Nel,Alice Burleigh,Ivan Doykov,Japhette Kembou-Ringert,Maximillian Woodall,Tereza Masonou,Katie-Marie Case,Arturo Torres Ortez,Timothy D McHugh,Antonio Casal,Laura E McCoy,Sudaxshina Murdan,Robert E Hynds,Kimberly C Gilmour,Louis Grandjean,Mario Cortina-Borja,Wendy E Heywood,Kevin Mills,Claire M Smith

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:727-736 PubMed34118143

2021

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Lei,Jingbin Shi,Yun Teng,Chenghui Song,Lijuan Zou,Fuxiu Ye,Haichen Zhang
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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