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AB234577

Human Vitronectin/S-Protein ELISA Kit

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Human Vitronectin/S-Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vitronectin/S-Protein with a sensitivity of 19.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 이미지
Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Interpolated concentrations of native Vitronectin in human serum samples.

The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 μg/mL in serum.

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Representative image of Human Vitronectin/S-Protein ELISA Kit (ab234577)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Vitronectin/S-Protein ELISA Kit Capture Antibody

- 10X Human Vitronectin/S-Protein Detector Antibody

- 10X Human Vitronectin/S-Protein ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Vitronectin/S-Protein ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.

The Vitronectin standard curve was prepared as described in Section 10.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 19.9 pg/mL

검출 범위

0.06 - 4 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Intra", "sample": "Recombinant Protein", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

Recovery

[ { "sample": "Serum", "range": "107 - 119.2 %", "average": "= 114.9" } ]

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234577-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234577-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234577-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB234577-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234577-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234577-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234577-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234577-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234577-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234577-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vitronectin/S-Protein Capture Antibody", "number":"AB234577-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Vitronectin/S-Protein Lyophilized Recombinant Protein", "number":"AB234577-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vitronectin/S-Protein Detector Antibody", "number":"AB234577-CMP03", "productcode":"" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
See full target information Vitronectin

대체 명칭 보기

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Theranostics 13:3897-3913 PubMed37441594

2023

Macrophage promotes fibroblast activation and kidney fibrosis by assembling a vitronectin-enriched microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yiling Peng,Li Li,Jingyue Shang,Haili Zhu,Jinlin Liao,Xue Hong,Fan Fan Hou,Haiyan Fu,Youhua Liu
제품이 사용된 논문 모두 보기
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