AB317552

Human Wnt11 ELISA Kit

SimpleStep
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Human Wnt11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Wnt11 with a sensitivity of 8.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

sELISA

Supplier Data

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Example of human Wnt11 standard curve. Background-subtracted data values (mean +/- SD, n=2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Interpolated concentration of native Wnt11 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20 µg/mL TF-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Interpolated concentration of native Wnt11 was measured in duplicate at different sample concentrations. Undiluted samples are 90 µg/mL TF-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Example of human Wnt11 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD, n=2) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.8 pg/mL

검출 범위

65.625 - 4200 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Wnt11 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Wnt11 protein in Cell Lysate. Quantitate Human Wnt11 with 8.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "106 - 109 %", "average": "= 107" } ]

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317552-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317552-CMP11" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317552-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317552-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB317552-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317552-CMP06" }, { "size":"20 x 1 Vial", "name":"Human Wnt11 Lyophilized Recombinant Protein", "number":"AB317552-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Wnt11 Capture Antibody", "number":"AB317552-CMP01" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317552-CMP05" }, { "size":"10 x 600 µL", "name":"10X Human Wnt11 Detector Antibody", "number":"AB317552-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317552-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317552-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317552-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317552-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317552-CMP08" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB317552-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317552-CMP06" }, { "size":"2 x 1 Vial", "name":"Human Wnt11 Lyophilized Recombinant Protein", "number":"AB317552-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Wnt11 Capture Antibody", "number":"AB317552-CMP01" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317552-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317552-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human Wnt11 Detector Antibody", "number":"AB317552-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317552-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317552-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317552-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317552-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317552-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317552-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Wnt11 Lyophilized Recombinant Protein", "number":"AB317552-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB317552-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Wnt11 Capture Antibody", "number":"AB317552-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317552-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Wnt11 Detector Antibody", "number":"AB317552-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317552-CMP10", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

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제품 프로토콜

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타겟 정보

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

대체 명칭 보기

Protein Wnt-11, WNT11

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Human Wnt11 ELISA Kit

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

Sandwich ELISA - Human Wnt11 ELISA Kit (AB317552)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise