AB317548

Influenza A Virus Hemagglutinin ELISA Kit

SimpleStep
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Influenza A Virus Hemagglutinin ELISA Kit is a single-wash 90-min Simplestep used to quantify Influenza A Virus Hemagglutinin with a sensitivity of 18.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Hemagglutinin, HA

2 이미지

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

sELISA

Supplier Data

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

Example of Influenza A Virus Hemagglutinin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

Example of Influenza A Virus Hemagglutinin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Urine, Saliva

Reacts with

Influenza A

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 18.6 pg/mL

검출 범위

101.563 - 6500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Influenza A Virus Hemagglutinin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemagglutinin protein in Cell culture media. Quantitate Influenza A Virus Hemagglutinin with 18.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

This kit was made against Influenza A H1N1/Peurto Rico/8/1934. Cross reactivity was tested for Influenza A H3N2 (A/Darwin/9/2021), Influenza A H5N1 (A/Egypt/N000/2015), and Influenza A H1N1 (A/California/07/2009). No cross reactivity was observed.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "88 - 104 %", "average": "= 94" }, { "sample": "Urine", "range": "95 - 102 %", "average": "= 100" }, { "sample": "Saliva", "range": "105 - 110 %", "average": "= 107" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Virus Hemagglutinin also known as hemagglutinin or influenza HA is a surface glycoprotein found on the influenza A virus. Hemagglutinin has a mass of approximately 75 kDa and serves as an important player in the virus's ability to infect host cells. It is expressed on the exterior membrane of the influenza virus and allows the virus to attach to and enter host cells initiating the infection process. This protein comes in various subtypes adding complexity to the virus's infection strategies and complicating vaccine development.

Biological function summary

Hemagglutinin facilitates the binding of the virus to sialic acid receptors on the surface of host epithelial cells. This binding triggers endocytosis allowing the virus entry into the host cell. Hemagglutinin is part of a complex that works closely with neuraminidase another glycoprotein on the virus surface. The hemagglutinin-neuraminidase complex is essential for the viral life cycle balancing attachment to host cells with the release of new viral particles.

Pathways

Hemagglutinin plays a significant role in the viral infection pathway of the influenza virus. It is critical in the initial steps of the pathway that involve viral entry and fusion with the host cell membrane. Hemagglutinin's interaction with the sialic acid receptors is closely linked with the activity of neuraminidase which aids in the release stage of the infection. This interplay between hemagglutinin and neuraminidase highlights their participation in the viral replication cycle demonstrating their complementary roles.

Hemagglutinin is deeply connected to seasonal influenza outbreaks and pandemics. Alterations in the hemagglutinin protein such as mutations and antigenic drifts or shifts can lead to new influenza strains with pandemic potential. The interaction of hemagglutinin with host immune responses and its capability to change allows the virus to evade immune detection. Neuraminidase inhibitors a class of antiviral drugs target enzymes closely related to hemagglutinin to prevent the spread of infection showcasing hemagglutinin's centrality in both infection and therapeutic intervention strategies.

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제품 프로토콜

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타겟 정보

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

See full target information HA

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websiteProtocolBooklet

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Product promise

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Influenza A Virus Hemagglutinin ELISA Kit

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

Sandwich ELISA - Influenza A Virus Hemagglutinin ELISA Kit (AB317548)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Primary Antibodies

Assay Kits

Assay Kits

Proteins & Peptides

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise