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AB318951

Kanamycin ELISA Kit - Intracellular

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Kanamycin ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Kanamycin with a sensitivity of 33.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
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Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Interpolated concentrations of spiked ​Kanamycin​ in chicken, pork, and shrimp extracts. ​​Kanamycin​ was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 1 mg/mL chicken extract, pork extract, and shrimp extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Interpolated concentrations of spiked ​Kanamycin​ in mouse kidney extract. ​​Kanamycin​ was spiked into the following sample and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted sample is 0.25 mg/mL mouse kidney extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)
  • cELISA

Supplier Data

Competitive ELISA - Kanamycin ELISA Kit - Intracellular (AB318951)

Example of ​Kanamycin​ standard curve. OD (450 nm) values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

분석 유형

Competitive

결과 유형

Quantitative

Sensitivity

= 33.7 pg/mL

검출 범위

93.75 - 6000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Kanamycin SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Kanamycin in Tissue Extracts. Quantitate Kanamycin with 33.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "96 - 108 %", "average": "= 103" } ]
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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318951-10x96Tests", "assetComponentDetails": [ { "size":"10 x 2 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318951-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB318951-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318951-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318951-CMP07" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB318951-CMP11" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318951-CMP05" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318951-CMP04" }, { "size":"10 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318951-CMP01" }, { "size":"10 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318951-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318951-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318951-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Kanamycin Lyophilized Standard", "number":"AB318951-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318951-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318951-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318951-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318951-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 4BR", "number":"AB318951-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318951-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin CaptSure Conjugate", "number":"AB318951-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Kanamycin HRP Conjugate", "number":"AB318951-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318951-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB318951-CMP11", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

See full target information Kanamycin
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websiteProtocolBooklet
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Product promise

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