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AB323738

Monkey Cystatin C ELISA Kit

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Monkey Cystatin C ELISA Kit is a Sandwich ELISA for the measurement of Monkey Cystatin C in Rhesus monkey in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)

Example of monkey Cystatin C standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)

Example of monkey Cystatin C standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)

Interpolated concentration of native Cystatin C was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 10,000, plasma (citrate) 1 : 5,000, plasma (EDTA) 1 : 5,000, and plasma (heparin) 1 : 5,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)

Interpolated concentration of native Cystatin C was measured in duplicate at different sample concentrations. Undiluted samples are as follows : PBMC supernatant 1 : 100 and cerebrospinal fluid 1 : 10,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Cystatin C ELISA Kit (AB323738)

Interpolated concentration of native Cystatin C was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 10,000 monkey serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Rhesus monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2 pg/mL

검출 범위

10.938 - 700 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Monkey Cystatin C SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Cerebral Spinal Fluid . Quantitate Monkey Cystatin C with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.5" } ]

Recovery

[ { "sample": "Serum", "range": "101 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "101 - 106 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "95 - 122 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "88 - 104 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "101 - 120 %", "average": "= 110" }, { "sample": "Cerebral Spinal Fluid", "range": "100 - 118 %", "average": "= 110" } ]

제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3

대체 명칭 보기

Cystatin-C, Cystatin-3, CST3
websiteProtocolBooklet
en

Product promise

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