AB317549

Monkey Eotaxin ELISA Kit

SimpleStep
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Monkey Eotaxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey Eotaxin with a sensitivity of 12.913 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput

2 이미지

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

sELISA

Supplier Data

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

Example of monkey Eotaxin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

Example of monkey Eotaxin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Citrate plasma

Reacts with

Monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 12.913 pg/mL

검출 범위

39.063 - 2500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Monkey Eotaxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin protein in Serum, EDTA Plasma, Cit Plasma. Quantitate Monkey Eotaxin with 12.913 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]

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Recovery

[ { "sample": "Serum", "range": "89 - 101 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "97 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "77 - 82 %", "average": "= 79" } ]

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제품 구성

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317549-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317549-CMP11" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB317549-CMP05" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317549-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317549-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317549-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB317549-CMP04" }, { "size":"2 x 1 Vial", "name":"Monkey Eotaxin Lyophilized Recombinant Protein", "number":"AB317549-CMP03" }, { "size":"1 x 600 µL", "name":"10X Monkey Eotaxin Capture Antibody", "number":"AB317549-CMP01" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317549-CMP12" }, { "size":"1 x 600 µL", "name":"10X Monkey Eotaxin Detector Antibody", "number":"AB317549-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317549-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317549-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB317549-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317549-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317549-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317549-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey Eotaxin Capture Antibody", "number":"AB317549-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey Eotaxin Lyophilized Recombinant Protein", "number":"AB317549-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB317549-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317549-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey Eotaxin Detector Antibody", "number":"AB317549-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317549-CMP10", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

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제품 프로토콜

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타겟 정보

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells. Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.

See full target information CCL11

대체 명칭 보기

SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Small-inducible cytokine A11

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey Eotaxin ELISA Kit

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise