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AB242230

Monkey IL-4 ELISA Kit

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(2 제품이 사용된 논문 )

Monkey IL-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-4 with a sensitivity of 2.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
4 이미지
Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Example of monkey IL-4 standard curve in Sample Diluent 75BP. The IL-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Comparison of native IL-4 in rhesus and cynomolgus stimulated peripheral blood mononuclear cell (PBMC) supernatant samples.

PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 95.34 pg/mL in neat stimulated rhesus PBMC supernatant and 47.30 pg/mL in neat stimulated cynomolgus PBMC supernatant. Values for unstimulated supernatants were undetectable in both rhesus and cynomolgus (not shown).

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Interpolated concentrations of native IL-4 in rhesus macaque stimulated peripheral blood mononuclear cell (PBMC) Supernatant samples.

Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 95.34 pg/mL in neat stimulated PBMC supernatant, and undetectable in unstimulated PBMC supernatant and naive cell culture media (not shown).

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)
  • sELISA

Unknown

Sandwich ELISA - Monkey IL-4 ELISA Kit (AB242230)

Example of monkey IL-4 standard curve in Sample Diluent NS. The IL-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey, Cynomolgus monkey, Rhesus monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.2 pg/mL

검출 범위

15.6 - 1000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Monkey IL-4 ELISA Kit (ab242230) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-4 with 2.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "115 - 133 %", "average": "= 121" }, { "sample": "Serum", "range": "75 - 81 %", "average": "= 79" }, { "sample": "EDTA Plasma", "range": "94 - 98 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "108 - 116 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "103 - 104 %", "average": "= 104" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242230-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB242230-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB242230-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB242230-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242230-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242230-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242230-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242230-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242230-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-4 Capture Antibody", "number":"AB242230-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey IL-4 Lyophilized recombinant Protein", "number":"AB242230-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-4 Detector Antibody", "number":"AB242230-CMP04", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4

대체 명칭 보기

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 30:59 PubMed38745316

2024

Polyphyllin I alleviates neuroinflammation after cerebral ischemia-reperfusion injury via facilitating autophagy-mediated M2 microglial polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Kang,Qiuling Sang,Dingxi Liu,Libo Wang,Jia Li,Xiaoyang Liu

International archives of allergy and immunology 183:375-388 PubMed34942624

2021

Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyun He,Yujuan Chen,Xiaoer Chen,Binfan Yuan
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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