AB326898
Monkey Interferon gamma ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- 각 태그의 뜻
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Monkey Interferon gamma ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Monkey Interferon gamma with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.97 - 24000 pg/ml with a sensitivity of 2.47 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.97 - 24000 pg/ml with a sensitivity of 2.47 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
3 이미지
- sELISA
Lab
Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
Dynamic range comparison between the Chemiluminescent Monkey Interferon gamma SimpleStep ELISA and the colorimetric Monkey Interferon gamma SimpleStep ELISA (ab315428). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range
- sELISA
Lab
Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
Dynamic range comparison between the Chemiluminescent Monkey Interferon gamma SimpleStep ELISA and the colorimetric Monkey Interferon gamma SimpleStep ELISA (ab315428). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
- sELISA
Supplier Data
Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
Interpolated concentrations of native Interferon gamma in cynomolgus monkey unstimulated and stimulated PBMC cell culture supernatant samples
Interpolated concentration of native Interferon gamma was measured in duplicate at different sample concentrations. Undiluted samples are as follows : unstimulated PBMC cell culture supernatant (50%) and stimulated PBMC cell culture supernatant (2.5%) treated with PMA (50 ng/mL) and ionomycin (1 µg/mL) for 24 hrs. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Monkey Interferon gamma ELISA Kit, Chemiluminescent (ab326898) is a rapid single-wash sandwich 55-min ELISA to measure Monkey Interferon gamma in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant with a sensitivity of 2.47 pg/mL.
How the assay works
Monkey Interferon gamma ELISA Kit, Chemiluminescent (ab326898) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Monkey Interferon gamma (ab326898) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Interferon gamma antibody [EPR24641-55] - BSA and Aside free (Capture) ab289984
- Anti-Interferon gamma antibody [EPR24641-20] - BSA and Aside free (Detector) ab289983
How the assay works
Monkey Interferon gamma ELISA Kit, Chemiluminescent (ab326898) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Monkey Interferon gamma (ab326898) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Interferon gamma antibody [EPR24641-55] - BSA and Aside free (Capture) ab289984
- Anti-Interferon gamma antibody [EPR24641-20] - BSA and Aside free (Detector) ab289983
Precision
[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" } ]
Recovery
[ { "sample": "Serum", "range": "91 - 98 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "95 - 106 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "96 - 109 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "109 - 112 %", "average": "= 110" }, { "sample": "Cerebral Spinal Fluid", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "108 - 115 %", "average": "= 110" } ]
제품 구성
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특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
타겟 정보
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
대체 명칭 보기
Interferon gamma, IFN-gamma, IFNG
Product promise
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