Monkey MCP1 ELISA Kit
Monkey MCP1 ELISA 키트
- Recombinant
- SimpleStep
- 각 태그의 뜻
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Monkey MCP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey MCP1 with a sensitivity of 2.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
MCP1, SCYA2, CCL2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1
- sELISA
Unknown
Sandwich ELISA - Monkey MCP1 ELISA Kit (AB242231)
Interpolated concentrations of native MCP1 in stimulated rhesus macaque peripheral blood monocyte cell (PBMC) supernatant samples.
Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 240.3 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC supernatant and naive cell culture media (not shown).
- sELISA
Unknown
Sandwich ELISA - Monkey MCP1 ELISA Kit (AB242231)
Example of monkey MCP1 standard curve in Sample Diluent 50BS. The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Monkey MCP1 ELISA Kit (AB242231)
Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours). The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Monkey MCP1 ELISA Kit (AB242231)
Interpolated concentrations of native MCP1 in rhesus macaque serum and plasma samples. The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 496.0 pg/mL in neat serum, 157.9 pg/mL in plasma (citrate), 198.0 pg/mL in neat plasma (heparin), and 169.7 pg/mL in neat plasma (EDTA).
- sELISA
Unknown
Sandwich ELISA - Monkey MCP1 ELISA Kit (AB242231)
Example of monkey MCP1 standard curve in Sample Diluent NS. The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Monkey MCP1 ELISA Kit (ab242231) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey MCP1 with 2.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
타겟 정보
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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