AB289698

Monkey MIP-1 alpha/CCL3 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Monkey MIP-1 alpha/CCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey MIP-1 alpha/CCL3 with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

2 이미지

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

sELISA

Supplier Data

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

Example of monkey MIP-1 alpha standard curve in Sample Diluent NS.

Example of monkey MIP-1 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

Interpolated concentrations of monkey MIP-1 alpha in PBMC stimulated cell culture supernatant.

Interpolated concentration of native MIP-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 PBMC stimulated cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in unstimulated.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.7 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Monkey MIP-1 aplha/CCL3) ELISA Kit (ab289698) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey MIP-1 alpha/CCL3 in serum, plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey MIP-1 alpha/CCL3 with 8.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 103 %", "average": "= 101" }, { "sample": "Serum", "range": "78 - 79 %", "average": "= 78" }, { "sample": "EDTA Plasma", "range": "97 - 108 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "80 - 85 %", "average": "= 82" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289698-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB289698-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289698-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289698-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB289698-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289698-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289698-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289698-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey MIP-1 alpha/CCL3 Detector Antibody", "number":"AB289698-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey MIP-1 alpha/CCL3 Lyophilized Recombinant Protein", "number":"AB289698-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey MIP-1 alpha/CCL3 Capture Antibody", "number":"AB289698-CMP07", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.

Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.

style

left-column

제품 프로토콜

style

left-column

타겟 정보

See full target information C-C motif chemokine 3

대체 명칭 보기

C C motif chemokine 3, CCL3_HUMAN, Chemokine (C C motif) ligand 3, Chemokine C C motif ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78 alpha, LD78-alpha(4-69), MIP 1A, MIP-1-alpha, MIP-1-alpha(4-69), Macrophage inflammatory protein 1-alpha, PAT 464.1, SCYA 3, SIS alpha, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, macrophage inflammatory protein 1a, small inducible cytokine A3 (homologous to mouse Mip-1a)

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey MIP-1 alpha/CCL3 ELISA Kit

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise