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AB325907

Mouse Adiponectin ELISA Kit, Chemiluminescent

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Mouse Adiponectin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Adiponectin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 9.14 - 20000 pg/ml with a sensitivity of 2.11 pg/mL.
- Patent pending
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Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)
  • sELISA

Lab

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)
  • sELISA

Lab

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)

Dynamic range comparison between the Chemiluminescent Mouse Adiponectin SimpleStep ELISA, colorimetric Mouse Adiponectin SimpleStep ELISA (ab226900), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)
  • sELISA

Lab

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)

Standard curve comparison between the Chemiluminescent Mouse Adiponectin SimpleStep ELISA and colorimetric Mouse Adiponectin SimpleStep ELISA (ab226900) in NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)

Interpolated concentrations of native Adiponectin in mouse cell culture supernatant samples. The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 386 pg/mL in lung supernatant.

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Adiponectin ELISA Kit, Chemiluminescent (AB325907)

Interpolated concentrations of native Adiponectin in mouse serum and plasma samples. The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 666, plasma (EDTA) 1 : 666, plasma (heparin) 1 : 666, and plasma (citrate) 1 : 666. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 2,212 ng/mL in serum, 2,168 ng/mL in plasma (EDTA), 2,129 ng/mL in plasma (heparin) and 1,845 ng/mL in plasma (citrate).

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.11 pg/mL

검출 범위

9.14 - 20000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse Adiponectin ELISA Kit, Chemiluminescent (ab325907) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Adiponectin in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant with a sensitivity of 2.11 pg/mL.

How the assay works

Mouse Adiponectin ELISA Kit, Chemiluminescent (ab325907) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Adiponectin (ab325907) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "106 - 109 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "93 - 105 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "102 - 116 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "109 - 114 %", "average": "= 112" }, { "sample": "Cell culture supernatant", "range": "99 - 106 %", "average": "= 102" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
See full target information Adipoq

대체 명칭 보기

Acdc, Acrp30, Apm1, Adipoq, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte-specific protein AdipoQ, ACRP30
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websiteProtocolBooklet
en
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Product promise

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