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AB108887

Mouse alpha Macroglobulin ELISA Kit

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Mouse alpha Macroglobulin ELISA Kit is a Sandwich ELISA for the measurement of Mouse alpha Macroglobulin in Mouse in Biofluids samples.

대체 명칭 보기

A2mp, A2m, Alpha-2-macroglobulin-P, Alpha-2-macroglobulin

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Sandwich ELISA - Mouse alpha Macroglobulin ELISA Kit (AB108887)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse alpha Macroglobulin ELISA Kit (AB108887)

Mouse alpha Macroglobulin ELISA Kit.

Typical standard curve using ab108887.

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Plasma, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.24 ng/mL

검출 범위

0.391 - 100 ng/mL

분석 소요 시간

4h

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum amd urine.

An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108887-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108887-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108887-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108887-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108887-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108887-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108887-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated alpha Macroglobulin Antibody", "number":"AB108887-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"alpha Macroglobulin Microplate (12 x 8 well strips)", "number":"AB108887-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha Macroglobulin Standard", "number":"AB108887-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Macroglobulin also known as alpha-2-Macroglobulin (A2M) is a large plasma glycoprotein with a molecular mass of approximately 725 kDa. It functions as a broad-spectrum protease inhibitor capable of trapping a wide range of proteases including serine cysteine aspartic and metalloproteases. A2M possesses a "bait region" that captures these proteases facilitating a conformational change that sequesters them. It is highly expressed in the liver and secreted into the bloodstream maintaining its presence at high levels in plasma.
Biological function summary

Alpha-2-Macroglobulin acts as an inhibitor of various proteases contributing to numerous physiological processes. A2M is not a part of a larger complex working independently to regulate protease activity. Its role in binding and inactivating proteases helps control inflammation and tissue degradation making it important for homeostasis. By capturing and neutralizing excess proteases in the blood A2M helps prevent potential tissue damage and regulates protein turnover.

Pathways

Alpha-2-Macroglobulin is involved in the protease inhibitor pathways and is linked to processes like the acute phase reaction. In the acute phase response A2M modulates protease activity maintaining balance in protease-antiprotease homeostasis. A2M interacts with various cytokines and growth factors such as Transforming Growth Factor-beta (TGF-beta) influencing their signaling processes and modulating immune responses.

Alpha-2-Macroglobulin is associated with conditions such as Alzheimer's disease and rheumatoid arthritis. In Alzheimer's disease A2M interacts with amyloid-beta peptide influencing its clearance and deposition in the brain. This interaction can affect disease progression and symptom severity. In rheumatoid arthritis A2M levels can change in response to inflammation impacting joint tissue degradation and immune system regulation often in conjunction with inflammatory mediators like Tumor Necrosis Factor-alpha (TNF-alpha).
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제품 프로토콜

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타겟 정보

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).
See full target information A2m
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websiteProtocolBooklet
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Product promise

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