Mouse Angiopoietin 2 ELISA Kit

제품 세부 정보

Mouse Angiopoietin 2 ELISA Kit (ab209883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin 2 with 17.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.