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AB264613

Mouse Annexin A1 ELISA Kit

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(3 제품이 사용된 논문 )

Mouse Annexin A1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Annexin A1 with a sensitivity of 0.144 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
2 이미지
Sandwich ELISA - Mouse Annexin A1 ELISA Kit (AB264613)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A1 ELISA Kit (AB264613)

Example of mouse Annexin A1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Annexin A1 ELISA Kit (AB264613)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A1 ELISA Kit (AB264613)

Interpolated concentrations of native Annexin A1 in mouse NIH/3T3 cell extract based on a 3.75 μg/mL extract load.

The concentrations of Annexin A1 were measured in duplicate and interpolated from the Annexin A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.144 ng/mL

검출 범위

0.195 - 12.5 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse Annexin A1 ELISA Kit (ab264613) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A1 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Annexin A1 with 0.13 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Annexin A1, also known as lipocortin I and Calpactin II, is a calcium dependent phospholipid binding protein encoded by the ANXA1 gene. Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "113 - 124 %", "average": "= 119" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264613-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB264613-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB264613-CMP15", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264613-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264613-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264613-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264613-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBS", "number":"AB264613-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264613-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264613-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264613-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Annexin A1 Lyophilized Recombinant Protein", "number":"AB264613-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Annexin A1 Capture Antibody", "number":"AB264613-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Annexin A1 Detector Antibody", "number":"AB264613-CMP11", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.
Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.
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제품 프로토콜

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타겟 정보

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 12475898). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (PubMed : 12475898). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T cells (PubMed : 17948261). Promotes the differentiation of T cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17948261). Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes (PubMed : 21245195). Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (PubMed : 21245195). In the context of antitumor immunity, interacts with FPR1 on dendritic cells allowing for tumor-associated antigens uptake and cross-presentation to T cells to mount an antitumor specific T cell response.. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2.
See full target information Anxa1

대체 명칭 보기

Anx1, Lpc-1, Lpc1, Anxa1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:1096 PubMed38321014

2024

Pathogenic NLRP3 mutants form constitutively active inflammasomes resulting in immune-metabolic limitation of IL-1β production.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Molina-López,Laura Hurtado-Navarro,Carlos J García,Diego Angosto-Bazarra,Fernando Vallejo,Ana Tapia-Abellán,Joana R Marques-Soares,Carmen Vargas,Segundo Bujan-Rivas,Francisco A Tomás-Barberán,Juan I Arostegui,Pablo Pelegrin

Biomolecules 12: PubMed35327639

2022

Inhibition of STAT6 Activation by AS1517499 Inhibits Expression and Activity of PPARγ in Macrophages to Resolve Acute Inflammation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Ji Lee,Kiyoon Kim,Minsuk Kim,Young-Ho Ahn,Jihee Lee Kang

Journal of neuroinflammation 18:119 PubMed34022892

2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Weiwei Gao,Lei Li,Jiheng Hao,Bin Yang,Tao Wang,Long Li,Xuesong Bai,Fanjian Li,Honglei Ren,Meng Zhang,Liyong Zhang,Jiyue Wang,Dong Wang,Jianning Zhang,Liqun Jiao
제품이 사용된 논문 모두 보기
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Product promise

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