Mouse Aspartate Aminotransferase ELISA Kit is a Sandwich ELISA for the measurement of Mouse Aspartate Aminotransferase in in Biofluids, Cell/Tissue Extracts samples.
대체 명칭 보기
Sialin, H(+)/nitrate cotransporter, H(+)/sialic acid cotransporter, Solute carrier family 17 member 5, Vesicular excitatory amino acid transporter, AST, VEAT, Slc17a5
- sELISA
Supplier Data
Sandwich ELISA - Mouse Aspartate Aminotransferase ELISA Kit (AB285300)
Standard AST Curve.
Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.
보완 제품
Primary Antibodies
AB134956
Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738]
RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127863.jpg)
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Reactivity 정보
제품 세부 정보
The Mouse AST ELISA kit (ab28530, E4320) is a sandwich ELISA assay for the quantitative measurement of AST in Mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Mouse AST captured from the samples.
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
This product is manufactured by BioVision, an Abcam company and was previously called E4320 Aspartate Aminotransferase (AST) (Mouse) ELISA Kit. E4320-100 is the same size as the 96 test size of ab285300.
Precision
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
타겟 정보
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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