AB240689

Mouse CCL9 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Mouse CCL9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL9 with a sensitivity of 1.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

sELISA

Unknown

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Interpolated concentrations of native CCL9 in mouse J774A.1 and RAW264.7 cell culture supernatant samples. The concentrations of CCL9 were measured in duplicates, interpolated from the CCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 cell culture supernatant 1 : 200 and RAW264.7 cell culture supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL9 concentration was determined to be 12.5 ng/ml in J774A.1 cell culture supernatant and 34.0 ng/ml in RAW264.7 cell culture supernatant.

sELISA

Unknown

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Example of mouse CCL9 standard curve in Sample Diluent NS. The CCL9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Interpolated concentrations of native CCL9 in mouse serum and plasma samples. The concentrations of CCL9 were measured in duplicates, interpolated from the CCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 200, plasma (heparin) 1 : 100, and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL9 concentration was determined to be 12.5 ng/mL in serum, 11.4 ng/mL in plasma (citrate), 13.8 ng/mL in plasma (heparin), and 14.2 ng/ml in plasma (EDTA).

sELISA

Unknown

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Mouse CCL9 Standard Curve Comparison. Standard Curve comparison between mouse CCL9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.03 pg/mL

검출 범위

2.2 - 140 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse CCL9 ELISA Kit (ab240689) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL9 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL9 with 1.03 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 97 %", "average": "= 91" }, { "sample": "Serum", "range": "84 - 98 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "95 - 97 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "86 - 104 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "85 - 92 %", "average": "= 88" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 1 gamma (MIP-1γ) also known as CCL9 functions as a chemokine involved in immune cell signaling. It weighs approximately 11 kDa. MIP-1γ attracts various immune cells to sites of inflammation within the body. The protein is expressed mainly in macrophages but you can also find it in other immune cells like dendritic cells. It plays a significant role in the body's response to inflammatory stimuli by mediating the recruitment of leukocytes.

Biological function summary

MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.

Pathways

MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.

MIP-1γ connects to various conditions including chronic inflammatory diseases and certain cancers. For example it has a link to rheumatoid arthritis where it contributes to the persistence of inflammation by recruiting immune cells to affected joints. It is also associated with some types of leukemia where its expression levels can influence disease progression. Proteins like interleukin-1β interact with MIP-1γ in these conditions as they both participate in inflammatory responses that exacerbate disease states.

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제품 프로토콜

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타겟 정보

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

See full target information Ccl9

대체 명칭 보기

Mrp2, Scya10, Scya9, Ccl9, C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 23:226 PubMed35222703

2022

Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Cuiyu Chu,Yuankun Huang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CCL9 ELISA Kit

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise