Mouse CD137 ELISA Kit
- Recombinant
- SimpleStep
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Mouse CD137 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CD137 with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
CD137, Cd137, Ila, Ly63, Tnfrsf9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB
- sELISA
Supplier Data
Sandwich ELISA - Mouse CD137 ELISA Kit (AB205577)
Example of CD137 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse CD137 ELISA Kit (AB205577)
Concentrations of CD137 in various untreated and treated cell culture supernatant samples.
EL4.IL2 cells were serum starved for 24 hours and then cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. Mouse spleen cells were cultured in the presence of 5 μg/mL LPS for 6 days. The cell culture supernatants were prepared. The concentrations of CD137 were measured in the diluted cell culture supernatant samples in duplicates, interpolated from the CD137 standard curve and corrected for sample dilution. Note that EL4.IL2 cell culture supernatant samples were analyzed neat, 2X and 4X diluted. Note that mouse spleen cell culture supernatant samples were analyzed 4X, 8X and 16X diluted. The interpolated, dilution factor-corrected values are plotted in pg of CD137 per mL of neat supernatant (mean +/- SD, n=3).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CD137 ELISA Kit (AB205577)
Concentrations of 4-1BB in various cell culture supernatant samples.
EL4.IL2 cells were cultured in the absence of serum for 3 days. NIH3T3, L929, D10G4.1 and Raw 264.7 cells were cultured in the presence of serum for 3 days. The cell culture supernatants were prepared. The concentrations of CD137 were measured in the diluted cell culture supernatant samples in duplicates, interpolated from the 4-1BB standard curve and corrected for sample dilution. Note that EL4.IL2 cell culture supernatant samples were analyzed neat and 2X diluted. Note that NIH/3T3 cell culture supernatant samples were analyzed neat. Note that L929 cell culture supernatant samples were analyzed neat, 2X and 4X diluted. Note that D10G4.1 cell culture supernatant samples were analyzed 2X, 4X and 8X diluted. Note that Raw 264.7 cell culture supernatant samples were analyzed 10X, 20X and 40X diluted. The interpolated, dilution factor-corrected values are plotted in pg of CD137 per mL of neat supernatant (mean +/- SD, n=1-3).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CD137 ELISA Kit (AB205577)
Interpolated concentrations of CD137 in mouse serum, plasma and cell culture supernatant samples.
The concentrations of CD137 were measured in duplicates, interpolated from the CD137 standard curves and corrected for sample dilution. Note that 1X Diluted serum and plasma samples were pre-diluted to 20%. Note that 1X Diluted Raw 264.7 day 3 cell culture supernatant samples were pre-diluted to 10%. The interpolated, dilution factor-corrected values are plotted in pg of CD137 per mL of neat sample (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CD137 ELISA Kit (AB205577)
Example of CD137 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity 정보
제품 세부 정보
Mouse CD137 ELISA Kit (ab205577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD137 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD137 with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD137 (also known as 4-1BB and Tumor necrosis factor receptor superfamily member 9 (TNRSF9)), a member of tumor necrosis factor receptor family, is a plasma membrane receptor for TNFSF9/4-1BBL. CD137 is expressed on the surface of activated T lymphocytes. CD137 is principally a homodimer, but also found as a monomer. CD137 generates either co-stimulatory signals leading to T cell activation and proliferation, or death signals, and may promote tumor rejection in vivo. Soluble forms of CD137 exist, these are generated by proteolytic cleavage or alternative splicing. In the years, functional expression of CD137 was noted on several non-T cells including monocytes, neutrophils, macrophages, B cells and natural killer (NK) cells.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.
Pathways
CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.
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