Mouse EGF ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
Be the first to review this product! 리뷰 제출
|
(3 제품이 사용된 논문 )
Mouse EGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse EGF with a sensitivity of 1.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
Pro-epidermal growth factor, EGF, Egf
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Interpolated concentrations of spike EGF in mouse serum and plasma samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native EGF concentration was determined to be 7.456 pg/mL in neat serum, 63.90 pg/mL in neat plasma (citrate), and not detectable in neat plasma (EDTA). Native data is not shown.
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Interpolated concentrations of native EGF in mouse kidney tissue extract based on a 400 μg/mL extract load.
The concentrations of EGF were measured in duplicate and interpolated from the EGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 90.16 pg/mL in mouse kidney tissue extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Example of mouse EGF standard curve in 1X Cell Extraction Buffer PTR.
The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Example of mouse EGF standard curve in Sample Diluent 25BS.
The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Interpolated concentrations of native EGF in mouse urine and kidney tissue supernatant samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 10,000 and kidney supernatant 1 : 4. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 600,860 pg/mL in neat urine and 384.1 pg/mL in neat kidney tissue supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Mouse EGF Competitor Std Curve Comparison.
Standard curve comparison between Mouse EGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity
- sELISA
Supplier Data
Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)
Example of mouse EGF standard curve in Sample Diluent NS.
The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Mouse EGF ELISA Kit (ab234560) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EGF protein in edta plasma, serum, tissue extracts, urine, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EGF with 1.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.
Pathways
EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.
타겟 정보
제품이 사용된 논문 (3)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nutrients 17: PubMed39861396
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cells 12: PubMed37408251
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com