AB157713
Mouse Ferritin ELISA Kit (FTL)
5
(2 리뷰들)
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(54 제품이 사용된 논문 )
Mouse Ferritin ELISA Kit (FTL) is a sandwich ELISA designed to quantify Mouse Ferritin (FTL) with a sensitivity of 3.1631 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 ng/mL
- Cited in over 40 publications
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 ng/mL
- Cited in over 40 publications
2 이미지
- sELISA
Supplier Data
Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (AB157713)
Representative standard curve using ab157713 Ferritin Mouse ELISA kit
- sELISA
Lab
Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (AB157713)
Ferritin measured in biologicals showing quantity (ng) per mL of tested sample
보완 제품
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Mouse Ferritin ELISA Kit (FTL) ab157713 is a sandwich ELISA to measure Mouse Ferritin (FTL) in serum, plasma, other biological fluids with a sensitivity of 3.1631 ng/ml.
How the assay works
In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Ferritin ELISA Kit (FTL) ab157713 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
5. Immediately begin recording the color development.
How the assay works
In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Ferritin ELISA Kit (FTL) ab157713 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
5. Immediately begin recording the color development.
Precision
[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": null, "average": "> 85" } ]
제품 구성
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특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
타겟 정보
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Degraded to release iron upon autophagy activation by nutrient starvation (PubMed : 25327288).
대체 명칭 보기
Ftl, Ftl-1, Ftl1, Ferritin light chain 1, Ferritin L subunit 1
제품이 사용된 논문 (54)
Recent publications for all applications. Explore the 전체 목록 and refine your search
JCI insight 10: PubMed40485584
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Development of rat and mouse models of heme-iron absorption.
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Jennifer K Lee,Yue He,Shireen Rl Flores,Regina R Woloshun,Xiaoyu Wang,Jacob S Shine,Pearl O Ebea-Ugwuanyi,Sitara Sriram,Melissa Fraga,Sean Zhu,Yang Yu,Iqbal Hamza,James F Collins
Amino acids 57:26 PubMed40355605
2025
Effect of histidine and carnosine on haemoglobin recovery in anaemia induced-kidney damage and iron-loading mouse models.
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Mayra Vera-Aviles,Jorge Moreno-Fernandez,Tugba Kose,Robert Hider,Gladys O Latunde-Dada
Journal of cancer research and clinical oncology 150:475 PubMed39441229
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A prospective diagnostic model for breast cancer utilizing machine learning to examine the molecular immune infiltrate in HSPB6.
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Lizhe Wang,Yu Wang,Yueyang Li,Li Zhou,Sihan Liu,Yongyi Cao,Yuzhi Li,Shenting Liu,Jiahui Du,Jin Wang,Ting Zhu
Blood advances 8:5753-5765 PubMed39250719
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In vivo silencing of intestinal DMT1 mitigates iron loading in β-thalassemia intermedia (Hbbth3/+) mice.
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Yang Yu,Regina R Woloshun,Jennifer K Lee,Pearl O Ebea-Ugwuanyi,Jacob S Shine,Sean Zhu,Yue He,James F Collins
Theranostics 14:3486-3508 PubMed38948064
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Deciphering mA methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy.
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Jiawen Li,Li Wei,Kaifeng Hu,Yunxiang He,Guidong Gong,Qisong Liu,Yue Zhang,Kaiyu Zhou,Junling Guo,Yimin Hua,Jun Tang,Yifei Li
Acta neuropathologica 147:106 PubMed38907771
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Ferroptosis inhibitor improves outcome after early and delayed treatment in mild spinal cord injury.
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Fari Ryan,Christian Blex,The Dung Ngo,Marcel A Kopp,Bernhard Michalke,Vivek Venkataramani,Laura Curran,Jan M Schwab,Klemens Ruprecht,Carolin Otto,Priya Jhelum,Antje Kroner,Samuel David
Disease models & mechanisms 17: PubMed38775430
2024
The NLRP3 inflammasome is essential for IL-18 production in a murine model of macrophage activation syndrome.
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Tara A Gleeson,Christina Kaiser,Catherine B Lawrence,David Brough,Stuart M Allan,Jack P Green
mBio 15:e0090524 PubMed38727220
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Helminth alleviates COVID-19-related cytokine storm in an IL-9-dependent way.
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Zengguo Cao,Jiaqi Wang,Xiaolei Liu,Yang Liu,Fangxu Li,Mingyuan Liu,Sandra Chiu,Xuemin Jin
Journal of radiation research 65:145-158 PubMed38247158
2024
Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo.
Applications
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Species
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Mamdooh Ghoneum,Nariman K Badr El-Din,Mai Alaa El-Dein
Endocrinology 164: PubMed37972265
2023
Fibroblast Growth Factor 23 Neutralizing Antibody Ameliorates Abnormal Renal Phosphate Handling in Sickle Cell Disease Mice.
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Species
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Liping Xiao,Kai Clarke,Marja M Hurley
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