AB325723

Mouse GPNMB ELISA Kit, Chemiluminescent

Recombinant
SimpleStep Ignite™ ELISA
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Mouse GPNMB ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse GPNMB with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 164.61 - 360000 pg/ml with a sensitivity of 46.27 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

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Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

sELISA

Lab

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Standard curve comparison between the Chemiluminescent Mouse GPNMB SimpleStep ELISA and colorimetric Mouse GPNMB SimpleStep ELISA (ab270892) in Sample Diluent 25BP. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Interpolated concentrations of native GPNMB in mouse serum and plasma samples. The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 19.4 ng/mL in neat serum, 6.5 ng/mL in plasma (citrate) and 6.6 ng/mL in plasma (EDTA).

sELISA

Lab

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Dynamic range comparison between the Chemiluminescent Mouse GPNMB SimpleStep ELISA, colorimetric Mouse GPNMB SimpleStep ELISA (ab270892) and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 46.27 pg/mL

검출 범위

164.61 - 360000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse GPNMB ELISA Kit, Chemiluminescent (ab325723) is a rapid single-wash sandwich 55-min ELISA to measure Mouse GPNMB in Serum, Citrate Plasma, EDTA Plasma, Cell Culture Media with a sensitivity of 46.27 pg/mL.

How the assay works

Mouse GPNMB ELISA Kit, Chemiluminescent (ab325723) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse GPNMB (ab325723) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Osteoactivin (Gpnmb) antibody [EPR22150-16] - BSA and Aside free (Capture) ab272356
- Anti-Osteoactivin (Gpnmb) antibody [EPR22150-10] - BSA and Aside free (Detector) ab272357

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "101 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "97 - 111 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "104 - 120 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "81 - 82 %", "average": "= 81" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325723-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325723-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GPNMB Detector Antibody", "number":"AB325723-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325723-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325723-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB325723-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325723-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325723-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GPNMB Capture Antibody", "number":"AB325723-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse GPNMB Lyophilized Recombinant Protein", "number":"AB325723-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB325723-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Could be a melanogenic enzyme.

See full target information Gpnmb

대체 명칭 보기

Dchil, Hgfin, Nmb, Gpnmb, Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse GPNMB ELISA Kit, Chemiluminescent

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

Sandwich ELISA - Mouse GPNMB ELISA Kit, Chemiluminescent (AB325723)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Proteins & Peptides

Assay Kits

Primary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise