JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327326

Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Heme Oxygenase 1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.57 - 10000 pg/ml with a sensitivity of 1.51 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
5 이미지
Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)

Quantitation of Heme Oxygenase 1 expression in different cell extracts.

Interpolated values of Heme Oxygenase 1 are plotted for the indicated cell extracts (y-axis) against the total extract load (x-axis).

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)

Interpolated concentrations of Heme Oxygenase 1 in mouse plasma (EDTA) plasma (heparin) and plasma (citrate).

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Heme Oxygenase 1 concentration was determined to be 5536 pg/mL in mouse plasma (EDTA) 6400 pg/mL in mouse plasma (heparin) and 5479 pg/mL in mouse plasma (Citrate).

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)

Interpolated concentrations of Heme Oxygenase 1 in mouse serum and splenocyte supernatant.

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Heme Oxygenase 1 concentration was determined to be 10670 pg/mL in mouse serum and 6084 pg/mL in mouse splenocyte supernatant.

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)
  • sELISA

Lab

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)

Standard curve comparison between the Chemiluminescent Mouse Heme Oxygenase 1 SimpleStep ELISA and colorimetric Mouse Heme Oxygenase 1 SimpleStep ELISA (ab204524) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)
  • sELISA

Lab

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (AB327326)

Dynamic range comparison between the Chemiluminescent Mouse Heme Oxygenase 1 SimpleStep ELISA, colorimetric Mouse Heme Oxygenase 1 SimpleStep ELISA (ab204524), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, EDTA Plasma, Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.51 pg/mL

검출 범위

4.57 - 10000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (ab327326) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Heme Oxygenase 1 in Serum, EDTA Plasma, Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 1.51 pg/mL.

How the assay works

Mouse Heme Oxygenase 1 ELISA Kit, Chemiluminescent (ab327326) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Heme Oxygenase 1 (ab327326) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Heme oxygenase 1 antibody [EPR18161-17] - BSA and Aside free (Capture) ab242480
- Anti-Heme oxygenase 1 antibody [EPR18161-140] - BSA and Aside free (Detector) ab242730

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "94 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "87 - 91 %", "average": "= 89" }, { "sample": "Urine", "range": "102 - 111 %", "average": "= 105" }, { "sample": "Tissue Extracts", "range": "104 - 113 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "87 - 108 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "108 - 119 %", "average": "= 112" } ]
style
left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327326-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Vial", "name":"Mouse Heme Oxygenase 1 Lyophilized Recombinant Protein", "number":"AB327326-CMP03" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327326-CMP11" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327326-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB327326-CMP04" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327326-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327326-CMP05" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327326-CMP10" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327326-CMP07" }, { "size":"10 x 600 µL", "name":"10X Mouse Heme Oxygenase 1 Detector Antibody", "number":"AB327326-CMP02" }, { "size":"10 x 600 µL", "name":"10X Mouse Heme Oxygenase 1 Capture Antibody", "number":"AB327326-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327326-CMP12" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327326-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327326-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327326-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327326-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327326-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Heme Oxygenase 1 Detector Antibody", "number":"AB327326-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327326-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Heme Oxygenase 1 Capture Antibody", "number":"AB327326-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327326-CMP12", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327326-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327326-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327326-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB327326-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Heme Oxygenase 1 Lyophilized Recombinant Protein", "number":"AB327326-CMP03", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (By similarity) Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (By similarity).. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
See full target information Hmox1

대체 명칭 보기

Heme oxygenase 1, HO-1, P32 protein, Hmox1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com