Mouse Hemopexin ELISA Kit (HPX)

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Mouse Hemopexin ELISA Kit (HPX) ab157716 is a sandwich ELISA to measure Mouse Hemopexin (HPX) in serum, plasma, other biological fluids with a sensitivity of 4.5092 ng/ml.

How the assay works

In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Hemopexin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemopexin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemopexin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemopexin in the test sample. The quantity of Hemopexin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Hemopexin ELISA Kit (HPX) ab157716 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development