AB324324

Mouse Heparanase 1 ELISA Kit

SimpleStep
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Mouse Heparanase 1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse Heparanase 1 in Mouse in Biofluids, Cell Culture Media samples.

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Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Example of mouse Heparanase 1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 2%, plasma citrate 25%, plasma EDTA 25%, and spleen supernatant 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : mouse serum 2% and rat serum 8%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Example of mouse Heparanase 1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 30.43 pg/mL

검출 범위

65.625 - 4200 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse Heparanase 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heparanase 1 protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Mouse Heparanase 1 with 30.43 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.2" } ]

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Recovery

[ { "sample": "Serum", "range": "101 - 102 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "84 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "92 - 101 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "94 - 96 %", "average": "= 95" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

See full target information Hpse

대체 명칭 보기

Hpa, Heparanase, Endo-glucoronidase

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websiteProtocolBooklet

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Product promise

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Mouse Heparanase 1 ELISA Kit

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise