AB325897

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent

Recombinant
SimpleStep Ignite™ ELISA
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Mouse HIF-1 alpha ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse HIF-1 alpha with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.29 - 22500 pg/ml with a sensitivity of 2.87 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

5 이미지

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Standard curve comparison between the Chemiluminescent Mouse HIF-1 alpha SimpleStep ELISA and colorimetric Mouse HIF-1 alpha SimpleStep ELISA (ab275103) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Interpolated concentrations of native HIF-1 alpha in mouse deferoxamine treated versus untreated NIH/3T3 cell extract based on a 10 µg/mL extract load. The concentrations of HIF1a were measured in duplicate, interpolated from the HIF1a standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF1a concentration was determined to be 485.55 pg/mL and 40.77 pg/ml in deferoxamine and mock treated cell extract, respectively.

sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Interpolated concentrations of native HIF-1 alpha in mouse NIH/3T3 cell extract treated with deferoxamine based on a 100 µg/mL extract load. The concentrations of HIF1a were measured in duplicate, interpolated from the HIF1a standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF1a concentration was determined to be 1,481 pg/mL.

sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Dynamic range comparison between the Chemiluminescent Mouse HIF-1 alpha SimpleStep ELISA, colorimetric Mouse HIF-1 alpha SimpleStep ELISA (ab275103), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell Extract, Tissue Extracts

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.87 pg/mL

검출 범위

10.29 - 22500 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (ab325897) is a rapid single-wash sandwich 55-min ELISA to measure Mouse HIF-1 alpha in Cell Extract, Tissue Extract with a sensitivity of 2.87 pg/mL.

How the assay works

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (ab325897) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse HIF-1 alpha (ab325897) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mouse-Hif1a antibody [EPR23071-183] - BSA and Aside free (Capture) ab275280
- Anti-Mouse-Hif1a antibody [EPR23071-113] - BSA and Aside free (Detector) ab275281

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 15" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "87 - 106 %", "average": "= 96" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed : 15225651, PubMed : 17981124, PubMed : 22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed : 15225651, PubMed : 17981124, PubMed : 22009797, PubMed : 22100406, PubMed : 37558878). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed : 22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed : 26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).

See full target information Hif1a

대체 명칭 보기

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Hif1a

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse HIF-1 alpha ELISA Kit, Chemiluminescent

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise