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AB326390

Mouse IgA ELISA Kit, Chemiluminescent

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Mouse IgA ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IgA with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.57 - 10000 pg/ml with a sensitivity of 1.17pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending
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Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)
  • sELISA

Lab

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)

Interpolated concentration of native IgA was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100000 serum plasma (citrate) plasma (EDTA) and plasma (heparin).  The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)
  • sELISA

Lab

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)

Standard curve comparison between the Chemiluminescent Mouse IgA SimpleStep ELISA and colorimetric Mouse IgA SimpleStep ELISA (ab314603) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)
  • sELISA

Lab

Sandwich ELISA - Mouse IgA ELISA Kit, Chemiluminescent (AB326390)

Dynamic range comparison between the Chemiluminescent Mouse IgA SimpleStep ELISA, colorimetric Mouse IgA SimpleStep ELISA (ab314603), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.17 pg/mL

검출 범위

4.57 - 10000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse IgA ELISA Kit, Chemiluminescent (ab326390) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IgA in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 1.17 pg/mL.

How the assay works

Mouse IgA ELISA Kit, Chemiluminescent (ab326390) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IgA (ab326390) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgA antibody [EPR25681-48] - BSA and Aside free (Capture) ab305148
- Anti-IgA antibody [EPR25681-94] - BSA and Aside free (Detector) ab305149
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.54" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "99 - 104 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "97 - 101 %", "average": "= 99" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
See full target information Ig alpha chain C region

대체 명칭 보기

Ig alpha chain C region
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websiteProtocolBooklet
en
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Product promise

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