AB46103

Mouse IL-10 ELISA Kit

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(13 제품이 사용된 논문 )

Mouse IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-10 in Mouse in Cell Culture Media, Biofluids samples.

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Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

Representative Standard Curve using ab46103

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 20 pg/mL

검출 범위

31.25 - 1000 pg/mL

분석 소요 시간

3h 45m

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's IL-10 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

This kit will recognize both endogenous and recombinant mouse IL-10.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "548.2 pg/mL", "standardDeviation": "12.1", "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "133.6 pg/mL", "standardDeviation": "5.4", "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "556.8 pg/mL", "standardDeviation": "11.6", "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "137.4 pg/mL", "standardDeviation": "6.8", "coefficientOfVariability": "4.9" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46103-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Murine IL-10 standard", "number":"AB46103-CMP05" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46103-CMP09" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46103-CMP04" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46103-CMP07" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46103-CMP02" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46103-CMP10" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46103-CMP03" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46103-CMP08" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46103-CMP06" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-10", "number":"AB46103-CMP01" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46103-2x96Tests", "assetComponentDetails": [ { "size":"4 x 1 Vial", "name":"Murine IL-10 standard", "number":"AB46103-CMP05", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46103-CMP03", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46103-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46103-CMP08", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46103-CMP02", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46103-CMP07", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46103-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46103-CMP10", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46103-CMP06", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-10", "number":"AB46103-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).

See full target information Il10

대체 명칭 보기

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

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제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

EBioMedicine 90:104480 PubMed36863256

2023

A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites.

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Yawei Wang,Binlin Tang,Huijuan Li,Jiancheng Zheng,Can Zhang,Zeyu Yang,Xu Tan,Peng Luo,Le Ma,Yang Wang,Lei Long,Zelin Chen,Zhenliang Xiao,Lijie Ma,Jing Zhou,Yu Wang,Chunmeng Shi

Journal of neuroinflammation 19:122 PubMed35624490

2022

Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway.

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Shuhui Dai,Jialiang Wei,Hongchen Zhang,Peng Luo,Yuefan Yang,Xiaofan Jiang,Zhou Fei,Wenbin Liang,Jianli Jiang,Xia Li

Cell death discovery 8:260 PubMed35568721

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microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis.

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Guifang Zhao,Hongquan Yu,Lijuan Ding,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling Qi

International journal of molecular sciences 20: PubMed31557796

2019

Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren's Syndrome-Like Disease.

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Ghada Abughanam,Osama A Elkashty,Younan Liu,Mohammed O Bakkar,Simon D Tran

International immunopharmacology 76:105835 PubMed31476692

2019

MicroRNA-124 alleviates the lung injury in mice with septic shock through inhibiting the activation of the MAPK signaling pathway by downregulating MAPK14.

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Species

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Weiyun Pan,Na Wei,Weiling Xu,Gang Wang,Fangchao Gong,Na Li

Journal of neuroinflammation 16:91 PubMed30995916

2019

Role of NADPH oxidase-2 in the progression of the inflammatory response secondary to striatum excitotoxic damage.

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Diego Rolando Hernández-Espinosa,Lourdes Massieu,Teresa Montiel,Julio Morán

Journal of immunology (Baltimore, Md. : 1950) 201:3199-3210 PubMed30373853

2018

FcγRIIb on B Cells and Myeloid Cells Modulates B Cell Activation and Autoantibody Responses via Different but Synergistic Pathways in Lupus-Prone Mice.

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Species

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Qingshun Lin,Mareki Ohtsuji,Hirofumi Amano,Hiromichi Tsurui,Norihiro Tada,Ryota Sato,Hidehiro Fukuyama,Hiroyuki Nishimura,J Sjef Verbeek,Sachiko Hirose

Probiotics and antimicrobial proteins 11:857-864 PubMed29682678

2018

More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer.

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Species

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Shahram Agah,Ali Mohammad Alizadeh,Maryam Mosavi,Peyman Ranji,Hossein Khavari-Daneshvar,Farnaz Ghasemian,Sahar Bahmani,Abbas Tavassoli

Frontiers in pharmacology 9:347 PubMed29695964

2018

Root Extract of Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin.

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Species

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Baohai Liu,Shuangdi Li,Xiaodan Sui,Lianyi Guo,Xingmei Liu,Hongmei Li,Leming Gao,Shusheng Cai,Yanrong Li,Tingting Wang,Xuehua Piao

Frontiers in cellular and infection microbiology 7:157 PubMed28536674

2017

Anti-Idiotypic Antibodies Specific to prM Monoantibody Prevent Antibody Dependent Enhancement of Dengue Virus Infection.

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Species

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Miao Wang,Fan Yang,Dana Huang,Yalan Huang,Xiaomin Zhang,Chao Wang,Shaohua Zhang,Renli Zhang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Mouse IL-10 ELISA Kit

Sandwich ELISA - Mouse IL-10 ELISA Kit (AB46103)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (13)

Product promise