AB236717

Mouse IL-12 p40 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Mouse IL-12 p40 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-12 p40 with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

대체 명칭 보기

Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, CLMF p40, Il12b

3 이미지

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Interpolated concentrations of native IL-12 p40 in mouse serum and plasma samples.

The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-12 p40 concentration was determined to be 1509 pg/mL in serum, 856 pg/mL in plasma (citrate), 822 pg/mL in plasma (EDTA) and 2285 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Interpolated concentrations of spiked IL-12 p40 in mouse cell culture media samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Example of mouse IL-12 p40 standard curve in Sample Diluent NS.

The IL-12 p40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

Sensitivity

= 3.7 pg/mL

검출 범위

15.6 - 1000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse IL-12 p40 ELISA Kit (ab236717) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-12 p40 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-12 p40 with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Serum", "range": "108 - 124 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "118 - 125 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "103 - 113 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "105 - 114 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "92 - 96 %", "average": "= 94" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236717-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236717-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236717-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236717-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB236717-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236717-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236717-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236717-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-12 p40 Capture Antibody", "number":"AB236717-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-12 p40 Lyophilized Recombinant Protein", "number":"AB236717-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-12 p40 Detector Antibody", "number":"AB236717-CMP03", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

See full target information Il12b

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제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-12 p40 ELISA Kit

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

Sandwich ELISA - Mouse IL-12 p40 ELISA Kit (AB236717)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise