AB253220

Mouse IL-19 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Mouse IL-19 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Mouse IL-19 with a sensitivity of 19.84 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326402

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Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

sELISA

Unknown

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

Interpolated concentrations of spike IL-19 in mouse, plasma and cell culture media samples.

The concentrations of IL-19 were measured in duplicates, interpolated from the IL-19 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

Representative image of Mouse IL-19 ELISA Kit (ab253220)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse IL-19 Capture Antibody

- 10X Mouse IL-19 Detector Antibody

- Mouse IL-19 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Unknown

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

Example of mouse IL-19 standard curve in Sample Diluent NS + Enhancer.

The IL-19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 19.84 pg/mL

검출 범위

46.88 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse IL-19 ELISA Kit (ab253220) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-19 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-19 with 19.84 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin 19 is a member of the IL-10 anti-inflammatory cytokine subfamily. Encoded by the IL-19 gene, this gene is found expressed in resting monocytes and B cells that plays important roles in inflammatory responses by binding the cell surface receptor IL-20R. In doing so, IL-19 upregulates IL-6 and TNF-alpha and induces apoptosis. Other stimulations such as granulocyte-macrophage colony-stimulating factor or lipopolysaccharide subsequently upregulates IL-19 in monocytes as well.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

Recovery

[ { "sample": "Serum", "range": "111 - 127 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "103 - 118 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "75 - 88 %", "average": "= 81" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253220-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB253220-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB253220-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253220-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253220-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253220-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253220-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253220-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253220-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-19 Detector Antibody", "number":"AB253220-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-19 Capture Antibody", "number":"AB253220-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-19 Lyophilized recombinant Protein", "number":"AB253220-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Cytokine that functions as an anti-inflammatory and proangiogenic factor (PubMed : 12370360, PubMed : 27053520). Polarizes adaptive immunity to an anti-inflammatory phenotype through induction of T-helper 2 responses by both down-regulation of IFN-gamma and up-regulation of IL4 and IL5 (PubMed : 15557163). Produced by osteocytes, stimulates granulopoiesis and neutrophil formation (PubMed : 33684929). Exerts its biological effect through a receptor complex consisting of a heterodimer of IL20RA and IL20RB. In turn, activates the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathway, and importantly, STAT3 (By similarity).

See full target information Il19

대체 명칭 보기

Interleukin-19, IL-19, Il19

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

The Journal of clinical investigation 132: PubMed36006737

2022

IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei He,Wenqian Luo,Yingjie Liu,Yuan Wang,Chengxin Ma,Qiuyao Wu,Pu Tian,Dasa He,Zhenchang Jia,Xianzhe Lv,Yu-Shui Ma,Haitang Yang,Ke Xu,Xue Zhang,Yansen Xiao,Peiyuan Zhang,Yajun Liang,Da Fu,Feng Yao,Guohong Hu

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-19 ELISA Kit

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

Sandwich ELISA - Mouse IL-19 ELISA Kit (AB253220)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise