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AB238258

Mouse IL-1RA ELISA Kit

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Mouse IL-1RA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-1RA with a sensitivity of 10.3 pg/well. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

4 이미지
ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • ELISA

Supplier Data

ELISA - Mouse IL-1RA ELISA Kit (AB238258)
Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Example of mouse IL1RA standard curve in Sample Diluent NS. The IL1RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Interpolated concentrations of native IL1RA in mouse serum and plasma samples. The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% plasma (citrate) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 200 pg/mL in serum, 216 pg/mL in plasma (citrate) and 488 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-1RA ELISA Kit (AB238258)

Interpolated concentrations of native IL1RA in mouse cell culture supernatant samples.

The concentrations of IL1RA were measured in duplicates, interpolated from the IL1RA standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 unstimulated 100%, and J774A.1 stimulated 12.5% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1RA concentration was determined to be 426 pg/mL in unstimulated J774A.1 and 8,581 pg/mL in stimulated J774A.1 cell culture supernatant.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 10.3 pg/well

검출 범위

46.88 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse IL-1RA ELISA Kit ab238258 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-1RA in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10.3 pg/weLL.

How the assay works

Mouse IL-1RA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-1RA ELISA Kit ab238258 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-1RA antibody [EPR17553-193] - BSA and Azide free (Capture) ab244793
- Anti-IL-1RA antibody [EPR17553-198] - BSA and Azide free (Detector) ab245066
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 108 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "93 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "112 - 126 %", "average": "= 121" }, { "sample": "Citrate plasma", "range": "95 - 106 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "114 - 120 %", "average": "= 116" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information Il1rn
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websiteProtocolBooklet
en
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Product promise

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