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AB171180

Mouse IL-21 ELISA Kit

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Mouse IL-21 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-21 in Mouse in Cell Culture Media, Biofluids samples.

대체 명칭 보기

Interleukin-21, IL-21, Il21

1 이미지
Sandwich ELISA - Mouse IL-21 ELISA Kit (AB171180)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB171180)

Representative standard curve using ab171180

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 10 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 플랫폼

Microplate

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's mouse IL-21 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse IL-21 in cell culture supernatants and serum.

An IL-21 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-21 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse IL-21 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171180-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171180-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171180-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171180-CMP08", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171180-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171180-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171180-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse IL-21 antibody Microplate (12 x 8 wells)", "number":"AB171180-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171180-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171180-CMP10", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse IL-21 standard", "number":"AB171180-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse IL-21 antibody", "number":"AB171180-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).
See full target information Il21
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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