Mouse IL-31 ELISA Kit

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The Mouse IL-31 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213872) is designed for the quantitative measurement of Mouse IL-31 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-31 amount of sample captured in plate.

Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL-31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL-31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL-31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL-31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.