AB222503

Mouse IL-6 ELISA Kit

Name: Mouse IL-6 ELISA 키트 (ab222503) | Abcam
Brand: Abcam Limited
SKU: AB222503
Rating: 5 (2 reviews)

Mouse IL-6 ELISA 키트

Recombinant
SimpleStep
각 태그의 뜻

(2 리뷰들)

(192 제품이 사용된 논문 )

Mouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility - works with serum, plasma, and cell culture supernatants
- Cited in over 130 publications

8 이미지

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve.

The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse serum and plasma samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution.

Native IL-6 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-6 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated RAW264.7 cell culture supernatant 1 : 50 and stimulated spleen cell culture supernatant 1 : 16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve. The IL-6 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 11.3 pg/mL

검출 범위

15.6 - 1000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse IL-6 ELISA Kit ab222503 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 11.3 pg/mL.

How the assay works

SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Mouse IL-6 ELISA Kit ab222503 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse IL-6 ELISA kit ab222503

Mouse IL-6 ELISA kit ab222503 has been used to assess inflammation in different physio-pathological contexts such as liver disease⁽¹⁾, diabetes⁽²⁾, and aging⁽³⁾.

References:

⁽¹⁾Ai-Lei Xu et al. 2024, PMID: 37902450,
⁽²⁾Lili Shi et al. 2024, PMID: 38169591,
⁽³⁾Yang Bao et al. 2023, PMID: 37954634

Save your precious samples

Our 384-well Mouse IL-6 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse IL-6 Antibody Pair - BSA and Azide free ab241663
- Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture) ab242490
- Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector) ab242739

Related and recommended products

Mouse IL-6 ELISA kit ab222503 is commonly used to measure IL-6 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-10 ELISA kit ab255729
- Mouse IL-1 beta ELISA kit ab197742

In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools

In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools

Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Spleen CCS", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Spleen CCS", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

Recovery

[ { "sample": "Serum", "range": "103 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "109 - 116 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "95 - 103 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "96 - 102 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "89 - 112 %", "average": "= 100" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

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제품 프로토콜

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타겟 정보

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (PubMed : 8910279). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (PubMed : 27893700).. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed : 16990136, PubMed : 27893700). Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).. Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed : 17075861). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed : 11786910). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed : 28402851). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (PubMed : 11113088). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed : 11113088, PubMed : 8910279). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed : 15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

See full target information Il6

대체 명칭 보기

Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1

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제품이 사용된 논문 (192)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecular medicine reports 32: PubMed40539450

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Protective effects of lipid mediators, obtained from docosahexaenoic acid via soybean lipoxygenase, on lipopolysaccharide‑induced acute lung injury through the NF‑κB and Nrf2/HO‑1 signaling pathways.

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Yan Su,Hack Sun Choi,Soon Kyu Kwon,Yunjon Han,Soon-Chang Cho,Jin Hyuk Shin,Yong-Suk Jang,Jong Hyun Choi,Jeong-Woo Seo

Journal of cell communication and signaling 19:e70019 PubMed40520073

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Role of circ_0012856 in modulating molecular pathways of diabetic peripheral neuropathy.

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Ji Chen,Fan Zhang,Yangyuxi Chen,Yingqing Lu,Xinxin Liu,Yuanzhang Tang,Fengrui Yang

Naunyn-Schmiedeberg's archives of pharmacology : PubMed40514518

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Alpha-asarone relieves nasal inflammation, epithelial barrier damage, and mitochondrial damage in allergic rhinitis by inhibiting mitochondrial ROS via the SIRT1/PGC-1α pathway.

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PloS one 20:e0322334 PubMed40323903

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Human cytomegalovirus-IE2 suppresses antigen presentation of macrophage through the IL10/STAT3 signalling pathway in transgenic mouse.

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Xianjuan Zhang,Qing Wang,Shuo Han,Guanghui Song,Bin Wang,Yunyang Wang

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Hepatic gluconeogenesis and PDK3 upregulation drive cancer cachexia in flies and mice.

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Isorhynchophylline Inhibits NLRP3 Inflammasome and Improves Gestational Diabetes.

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Cell biology and toxicology 41:59 PubMed40080209

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Exosome-mediated effects of BRCA1 on cardiovascular artery disease.

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Journal of thoracic disease 17:623-640 PubMed40083491

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Novel therapeutic strategy: Nrf2 activation in targeting senescence-related changes in chronic obstructive pulmonary disease.

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제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Mouse IL-6 ELISA Kit

Mouse IL-6 ELISA 키트

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (192)

Product promise