AB285341

Mouse Insulin ELISA Kit

Mouse Insulin ELISA 키트

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Mouse Insulin ELISA Kit is a sandwich ELISA designed to quantify Mouse Insulin with a sensitivity of 46.9 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78 - 5000 pg/mL

대체 명칭 보기

Ins-1, Ins1, Insulin-1

1 이미지

Sandwich ELISA - Mouse Insulin ELISA Kit (AB285341)

sELISA

Supplier Data

Sandwich ELISA - Mouse Insulin ELISA Kit (AB285341)

Mouse Insulin ELISA Kit - Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 46.9 pg/mL

검출 범위

78 - 5000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse Insulin ELISA Kit ab285341 is a sandwich ELISA to measure Mouse Insulin in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, other biological fluids with a sensitivity of 46.9 pg/ml.

How the assay works

The density of color is proportional to the amount of human Insulin captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Insulin ELISA Kit ab285341 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called K4271 Insulin (Mouse) ELISA Kit. K4271-100 is the same size as the 96 test size of ab285341.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

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제품 프로토콜

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타겟 정보

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information Ins1

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cell and developmental biology 13:1523266 PubMed40313719

2025

Genitourinary defects, anxiety and aggressive-like behavior and glucose metabolism disorders in mutant mice with inserted piggyBac transposon.

Applications

Unspecified application

Species

Unspecified reactive species

Rufeng Dai,Ye Yin,Minghui Yu,Yumeng Zhang,Jingjia Zhang,Tianyi Liu,Xiaoyan Fang,Xiaohui Wu,Qian Shen,Hong Xu

International journal of molecular sciences 25: PubMed39063084

2024

The Influence of BMP6 on Serotonin and Glucose Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Milešević,Ivona Matić Jelić,Viktorija Rumenović,Natalia Ivanjko,Slobodan Vukičević,Tatjana Bordukalo-Nikšić

Nature communications 14:6384 PubMed37821436

2023

Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Xin Liu,Li Han,Chenxu Ge,Yujie Zhang,Fufang Luo,Zhongqin Wang,Xiaoqin Xue,Liangyin Xiong,Xin Wang,Qinqin Zhang,Xiaoxin Wang,Qin Tian,Shuguang Zhang,Qingkun Meng,Xianling Dai,Qin Kuang,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Chunxiao Chang,Bochu Wang,Jie Chai,Shengbin Shi,Lianyi Han

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302130 PubMed37544908

2023

Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Yi Zhang,Fufeng Gao,Xianling Dai,Qin Kuang,Jie Chai,Benkui Zou,Bochu Wang

Acta pharmaceutica Sinica. B 13:1071-1092 PubMed36970206

2023

The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis blocking inactive rhomboid protein 2-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Wei Dong,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Jing Feng,Bochu Wang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Insulin ELISA Kit

Mouse Insulin ELISA 키트

Sandwich ELISA - Mouse Insulin ELISA Kit (AB285341)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise