Mouse Leptin R ELISA Kit is a Sandwich ELISA for the measurement of Mouse Leptin R in Mouse in Biofluids, Cell Culture Media samples.
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CD 295, CD295 antigen, Db, FA, HuB219, LEPRD, LEPR_HUMAN, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein, OB receptor, OB-R, OB-RGRP, obl
- sELISA
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Sandwich ELISA - Mouse Leptin R ELISA Kit (AB267584)
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Mouse Leptin R ELISA Kit is designed for the quantitative determination of Leptin R in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse Leptin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Leptin R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 4-1BB (TNFRSF9/CD137), Ace, ALK-1, Cardiotrophin-1 (CT-1), CD27 (TNFRSF7), CD32a, CD40 Ligand (TNFSF5), CTLA-4 (CD152), Decorin, DKK-1, Dtk, Endoglin (CD105), Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, Gas 6, GITR Ligand (TNFSF18), HAI-1, HGFR, IL-1 R4 (ST2), IL-3 R beta, IL-9, JAM-A (CD321/F11R), L-Selectin (CD62L), Lymphotactin (XCL1), MAdCAM-1, MFG-E8, MIP-3 beta (CCL19), Neprilysin, Pentraxin-3 (TSG-14), RAGE, TACI (TNFRSF13B), TREM-1, TROY (TNFRSF19), TSLP, TWEAK R (TNFRSF12), VEGFR1, VEGFR3.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.
Pathways
The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410831 PubMed39792613
2025
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