AB235648

Mouse LH Beta ELISA Kit

Name: Mouse LH Beta ELISA Kit
Brand: Abcam Limited
SKU: AB235648
Rating: 4 (1 reviews)

Recombinant
SimpleStep
각 태그의 뜻

(1 리뷰)

(2 제품이 사용된 논문 )

Mouse LH Beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LH Beta with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 이미지

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Example of mouse LH Beta standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Interpolated concentrations of native LH Beta in mouse pituitary extract based on a 6.25 μg/mL extract load.

The concentrations of LH Beta were measured in duplicate and interpolated from the LH Beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LH Beta concentration was determined to be 1.84 ng/mL in mouse pituitary extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Representative image of Mouse LH Beta ELISA Kit (ab235648)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse LH Beta Capture Antibody

- 10X Mouse LH Beta Detector Antibody

- Mouse LH Beta Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4.7 pg/mL

검출 범위

117 - 7500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse LH Beta ELISA Kit (ab235648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LH Beta protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LH Beta with 4.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Pituitary", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": null, "average": "= 97" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235648-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB235648-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB235648-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235648-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235648-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB235648-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235648-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB235648-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235648-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235648-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LH Beta Capture Antibody", "number":"AB235648-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse LH Beta Lyophilized Recombinant Protein", "number":"AB235648-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LH Beta Detector Antibody", "number":"AB235648-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.

Biological function summary

LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.

Pathways

The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.

Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.

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제품 프로토콜

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타겟 정보

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.

See full target information Lhb

대체 명칭 보기

Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, Lhb

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedicines 13: PubMed40426904

2025

Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ru Tsai,Yen-Nung Liao,Cheng-Ju Tsai,Yu-Ang Lee,Shih-Min Hsia,Kuo-Chung Lan,Hong-Yo Kang

Reproductive biology and endocrinology : RB&E 19:135 PubMed34496874

2021

Irisin alleviates obesity-related spermatogenesis dysfunction via the regulation of the AMPKα signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Huang-Guan Dai,Ling-Bo Luo,Jing Yang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse LH Beta ELISA Kit

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise