Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Standard curve comparison between the Chemiluminescent Mouse LIX SimpleStep ELISA and colorimetric Mouse LIX SimpleStep ELISA (ab264611) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Lab

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Dynamic range comparison between the Chemiluminescent Mouse LIX SimpleStep ELISA, colorimetric Mouse LIX SimpleStep ELISA (ab264611), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

• sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse serum, and plasma samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 20.21 ng/mL in serum, 1.37 ng/mL in plasma (citrate), 3.49 ng/mL in plasma (EDTA), and 5.04 ng/mL in plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in stimulated vs unstimulated mouse tissue culture supernatant samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 100%, LPS stimulated lung supernatant 1%, spleen supernatant 100%, and LPS stimulated spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 177.56 pg/mL in lung supernatant, 43.12 ng/mL LPS stimulated lung supernatant, 23.65 pg/mL spleen supernatant and 1250 pg/mL LPS stimulated spleen supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse LPS stimulated lung culture supernatant and LPS stimulated spleen culture supernatant samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : LPS stimulated lung culture supernatant 1% and LPS stimulated spleen culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 43.12 ng/mL in LPS stimulated lung supernatant and 1.25 ng/mL in LPS stimulated spleen supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse lung, spleen, and bone marrow based on a 500 μg/mL extract load.

The concentrations of LIX were measured in duplicate and interpolated from the LIX standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 571.73 pg/mL in lung extract, 548.73 pg/mL in spleen extract and 353.24 pg/mL in bone marrow extract.