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AB155449

Mouse MIP-1 gamma ELISA Kit (CCL9)

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(2 제품이 사용된 논문 )

Mouse MIP-1 gamma ELISA Kit (CCL9) is a Sandwich ELISA for the measurement of Mouse MIP-1 gamma (CCL9) in Mouse in Biofluids, Cell Culture Media samples.

대체 명칭 보기

Mrp2, Scya10, Scya9, Ccl9, C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2

2 이미지
Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)

Representative standard curve using ab155449 - Assay Diluent B.

Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)

Representative standard curve using ab155449 - Assay Diluent A.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 1 pg/mL

검출 범위

1 - 250 pg/mL

분석 플랫폼

Microplate

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보완 제품

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Human MIP-3 alpha ELISA Kit (CCL20)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's MIP-1 (CCL9) gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MIP-1 gamma in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MIP-1 (CCL9 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "95 - 103 %", "average": "= 99.02" }, { "sample": "Plasma", "range": "81 - 101 %", "average": "= 90.1" }, { "sample": "Serum", "range": "75 - 91 %", "average": "= 83.5" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155449-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155449-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155449-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155449-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIP-1 gamma Microplate (12 x 8 wells)", "number":"AB155449-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155449-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155449-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MIP-1 gamma Standard (lyophilized)", "number":"AB155449-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse MIP-1 gamma", "number":"AB155449-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155449-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 1 gamma (MIP-1γ) also known as CCL9 functions as a chemokine involved in immune cell signaling. It weighs approximately 11 kDa. MIP-1γ attracts various immune cells to sites of inflammation within the body. The protein is expressed mainly in macrophages but you can also find it in other immune cells like dendritic cells. It plays a significant role in the body's response to inflammatory stimuli by mediating the recruitment of leukocytes.
Biological function summary

MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.

Pathways

MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.

MIP-1γ connects to various conditions including chronic inflammatory diseases and certain cancers. For example it has a link to rheumatoid arthritis where it contributes to the persistence of inflammation by recruiting immune cells to affected joints. It is also associated with some types of leukemia where its expression levels can influence disease progression. Proteins like interleukin-1β interact with MIP-1γ in these conditions as they both participate in inflammatory responses that exacerbate disease states.
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제품 프로토콜

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타겟 정보

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
See full target information Ccl9
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Viruses 15: PubMed37515181

2023

NLRP3 Inflammasome Involved with Viral Replication in Cytopathic NADL BVDV Infection and IFI16 Inflammasome Connected with IL-1β Release in Non-Cytopathic NY-1 BVDV Infection in Bovine Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Gallegos-Rodarte,Omar Escobar-Chavarría,Meztli Miroslava Cantera-Bravo,Rosa Elena Sarmiento-Silva,Alejandro Benitez-Guzman

Journal of immunology research 2022:7200379 PubMed35465352

2022

Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Dang Sheng,Sui Xin,Wu Dongxing,Wen Shubo,Song Yang,Chen Zeliang,Zhai Jingbo
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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