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AB215538

Mouse PCSK9 ELISA Kit

Mouse PCSK9 ELISA 키트

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(7 제품이 사용된 논문 )

Mouse PCSK9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PCSK9 with a sensitivity of 2.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • ELISA

Supplier Data

ELISA - Mouse PCSK9 ELISA Kit (AB215538)
Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Interpolated concentrations of native PCSK9 in mouse serum and plasmas, and spiked recombinant mouse PCSK9 in cell culture supernatant.

The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.25%, plasma (heparin) 0.13% and RAW 264.7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 353 ng/mL in serum, 396 ng/mL in plasma (citrate), 348 ng/mL in plasma (EDTA), and 495 ng/mL in plasma (heparin). Mouse PCSK9 was not detected in unspiked 50% RAW 264.7 cell culture supernatant.

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Interpolated concentrations of spiked recombinant PCSK9 in mouse liver extract and RAW 264.7 cell extract based on a 1,000 μg/mL extract load.

The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. The interpolated, dilution-factor-corrected values are plotted (mean +/- SD, n=2). The mean native PCSK9 concentration was determined to be 2.36 ng/mg in liver extract and it was undetected in RAW 264.7 cell extract.

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Representative image of Mouse PCSK9 ELISA Kit (ab215538)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse PCSK9 Detector Antibody

- Mouse PCSK9 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse PCSK9 Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Example of mouse PCSK9 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Example of mouse PCSK9 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Interpolated concentration of native PCSK9 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 160 mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit (AB215538)

Example of mouse PCSK9 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.5 pg/mL

검출 범위

55 - 3500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (6 x 8 well strips)

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보완 제품

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Mouse AGT ELISA Kit

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AB230932

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Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse PCSK9 ELISA Kit ab215538 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse PCSK9 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 2.5 pg/mL.

How the assay works

Mouse PCSK9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse PCSK9 ELISA Kit ab215538 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PCSK9 antibody [EPR17827-111] - BSA and Azide free (Capture) ab242847
- Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (Detector) ab242847

PCSK9 (proprotein convertase subtilisin kexin 9), also called proprotein convertase 9 or NARC-1 (neural apoptosis-regulated convertase 1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. Mouse PCSK9 is encoded by the PCSK9 gene as a 694 amino acid enzyme that includes a signal peptide, a pro-domain, and a catalytic domain. PCSK9 undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain. Active PCSK9 may undergo additional N-terminal proteolysis by furin or proprotein convertase 5/6A, creating an inactive 53 kDa form. PCSK9 is involved in the regulation of plasma cholesterol homeostasis by mediating the degradation of low density lipoprotein receptor.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 105 %", "average": "= 98" }, { "sample": "Serum", "range": "102 - 107 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "101 - 102 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "128 - 130 %", "average": "= 129" }, { "sample": "Tissue Extracts", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "106 - 114 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 99" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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제품 프로토콜

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타겟 정보

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information Pcsk9

대체 명칭 보기

Narc1, Pcsk9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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