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AB213883

Mouse PLTP ELISA Kit

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Mouse PLTP ELISA Kit is a Sandwich ELISA for the measurement of Mouse PLTP in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

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Phospholipid transfer protein, Lipid transfer protein II, Pltp

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Sandwich ELISA - Mouse PLTP ELISA Kit (AB213883)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PLTP ELISA Kit (AB213883)

Mouse PLTP ELISA Kit (ab213883) Standard Curve.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 20 pg/mL

검출 범위

0.78 - 50 ng/mL

분석 소요 시간

3h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB300085

Anti-APOA1 antibody [EPR25446-9]

RabMAb|20ul selling size|Recombinant|KO Validated|Trial Size

Western blot - Anti-APOA1 antibody [EPR25446-9] (AB300085)

  • 0
  • 0
Primary Antibodies

AB282456

Anti-PLTP antibody [EPR24448-21]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLTP antibody [EPR24448-21] (AB282456)

  • 0
  • 0
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB275377

Anti-ALIX antibody [EPR23653-32]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ALIX antibody [EPR23653-32] (AB275377)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

The Mouse PLTP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213883) is designed for the quantitative measurement of Mouse PLTP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E18- A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PLTP amount of sample captured in plate.

Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "4.212 ng/mL", "standardDeviation": "0.32", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "21.284 ng/mL", "standardDeviation": "1.213", "coefficientOfVariability": "= 5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.296 ng/mL", "standardDeviation": "0.099", "coefficientOfVariability": "= 7.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "22.189 ng/mL", "standardDeviation": "1.02", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.418 ng/mL", "standardDeviation": "0.087", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "4.309 ng/mL", "standardDeviation": "0.284", "coefficientOfVariability": "= 6.6" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213883-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213883-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213883-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213883-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213883-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213883-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213883-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213883-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse PLTP coated Microplate (12 x 8 wells)", "number":"AB213883-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213883-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Mouse PLTPstandard", "number":"AB213883-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Mouse PLTP antibody", "number":"AB213883-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PLTP also known as Phospholipid Transfer Protein is an important component in human lipid metabolism. This protein functions by transferring phospholipids between different types of lipoproteins in plasma. PLTP possesses a molecular mass of about 56 kDa. Its expression occurs mainly in the liver and adipose tissue with additional presence in the brain and lungs. These locales of expression facilitate its key roles in lipid processing and lipoprotein metabolism.
Biological function summary

PLTP plays a significant role in remodeling high-density lipoproteins (HDL) and regulating lipoprotein particles. PLTP exists as a part of a larger complex involved in these processes interacting closely with other proteins in the lipoprotein maturation and transformation. This activity of PLTP significantly affects the levels and sizes of HDL particles in circulation thereby influencing cholesterol homeostasis within the body.

Pathways

PLTP acts within the reverse cholesterol transport pathway important for the removal of excess cholesterol from peripheral tissues. The protein works alongside Apolipoprotein A-I (ApoA-I) and Lecithin-Cholesterol Acyltransferase (LCAT) to promote cholesterol efflux and HDL maturation. Another key pathway involving PLTP is the lipid metabolism pathway where it influences lipid levels and impacts the function of other proteins such as Apolipoprotein E (ApoE).

PLTP has associations with atherosclerosis and metabolic syndrome. Variations in PLTP activity or expression can lead to altered HDL levels increasing the risk for heart disease and related cardiovascular conditions. In atherosclerosis PLTP's relationship with ApoA-I becomes especially relevant as this interaction influences lipid transport dynamics and plaque formation. Additionally in metabolic syndrome abnormal PLTP function may contribute to dyslipidemia a common factor in metabolic disorders.
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제품 프로토콜

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타겟 정보

Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves (PubMed : 10079112, PubMed : 7615508, PubMed : 7654777). Facilitates the transfer of a spectrum of different lipid molecules, including sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, and phosphatidyl ethanolamine (PubMed : 10079112). Plays an important role in HDL remodeling which involves modulating the size and composition of HDL (By similarity). Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion (By similarity). Two distinct forms of PLTP exist in plasma : an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability (By similarity).
See full target information Pltp
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Product promise

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