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AB315297

Mouse PSAP ELISA Kit

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Mouse PSAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PSAP with a sensitivity of 13.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Interpolated concentrations of mouse prosaposin in NIH/3T3 cell culture supernatant

Interpolated concentration of native prosaposin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20 NIH/3T3 cell culture supernatant.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Interpolated concentrations of mouse prosaposin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native prosaposin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Example of mouse prosaposin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 13.4 pg/mL

검출 범위

43.75 - 2800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse PSAP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSAP protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse PSAP with 13.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 116 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "91 - 102 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "96 - 100 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "88 - 92 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "91 - 103 %", "average": "= 97" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Prosaposin. Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.. Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).. Saposins are specific low-molecular mass non-enzymatic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
See full target information Psap

대체 명칭 보기

Sgp1, Psap, Prosaposin, Sulfated glycoprotein 1, SGP-1
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websiteProtocolBooklet
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Product promise

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