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AB325629

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent

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Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse/Rat ACTH (Adrenocorticotropic Hormone) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 2.06 - 1500 pg/ml with a sensitivity of 1.35 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending
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Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Standard curve comparison between the Chemiluminescent Mouse/Rat ACTH SimpleStep ELISA and colorimetric Mouse/Rat ACTH SimpleStep ELISA (ab263880) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Dynamic range comparison between the Chemiluminescent Mouse/Rat ACTH SimpleStep ELISA and colorimetric Mouse/Rat ACTH SimpleStep ELISA (ab263880), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Interpolated concentrations of native ACTH in mouse serum and plasma samples. The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 123 pg/mL in serum, 433 pg/mL in plasma (citrate), 240 pg/mL in plasma (heparin) and 328 pg/mL in plasma (EDTA).

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Interpolated concentrations of native ACTH in rat serum and plasma samples. The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 15%, plasma (heparin) 15% and plasma (EDTA) 15%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 1,178 pg/mL in serum, 2,975 pg/mL in plasma (citrate), 1,988 pg/mL in plasma (heparin) and 1,508 pg/mL in plasma (EDTA).

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Mouse, Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.35 pg/mL

검출 범위

2.06 - 1500 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Assay Kits

AB263880

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

Recombinant|SimpleStep

ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

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AB267814

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Recombinant|SimpleStep

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AB212166

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  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (ab325629) is a rapid single-wash sandwich 55 min ELISA to measure Mouse/Rat ACTH (Adrenocorticotropic Hormone) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 1.35 pg/mL.

How the assay works

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (ab325629) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse/Rat ACTH (Adrenocorticotropic Hormone) (ab325629) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture) ab259687
- Anti-ACTH antibody [EPR20361-235] - BSA and Azide free (Detector) ab259688

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.6" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 92 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "89 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "113 - 120 %", "average": "= 116" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

See full target information ACTH

대체 명칭 보기

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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