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AB324136

Mouse/Rat ADAMTS1 ELISA Kit- Intracellular

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Mouse/Rat ADAMTS1 ELISA Kit- Intracellular is a Sandwich ELISA for the measurement of Mouse/Rat ADAMTS1 - Intracellular in Mouse, Rat in Cell/Tissue Extracts samples.
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Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (AB324136)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (AB324136)

Interpolated concentration of native ADAMTS1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : NIH/3T3 cell extract 125 µg/mL, mouse kidney tissue extract 1,000 µg/mL, and rat lung tissue extract 1,000 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (AB324136)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (AB324136)

Example of mouse/rat ADAMTS1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Mouse, Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 51.27 pg/mL

검출 범위

140.625 - 9000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse/Rat ADAMTS1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ADAMTS1 protein in Cell Lysate, Tissue extracts. Quantitate Mouse/Rat ADAMTS1 with 51.27 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Singlewash protocol reduces assay time to 90 minutes or less.
  • High sensitivity, specificity and reproducibility from superior antibodies.
  • Fully validated in biological samples.
  • 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "90 - 97 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "98 - 106 %", "average": "= 101" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Metalloprotease which cleaves aggrecan, a cartilage proteoglycan, at the '1691-Glu-|-Leu-1692' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover (PubMed : 10930576, PubMed : 9441751). Also cleaves COMP (By similarity). Has angiogenic inhibitor activity (By similarity). May play a critical role in follicular rupture (PubMed : 10781075).
See full target information A disintegrin and metalloproteinase with thrombospondin motifs 1

대체 명칭 보기

A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, Adamts1
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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