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AB243685

Mouse RGM-C ELISA Kit

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Mouse RGM-C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse RGM-C with a sensitivity of 16.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)
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Supplier Data

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)

Mouse RGM-C Standard Curve Comparison.

Standard Curve comparison between mouse RGM-C SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)

Interpolated concentrations of native RGM-C in mouse serum and plasma samples.

The concentrations of RGM-C were measured in duplicates, interpolated from the RGM-C standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasm (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGM-C concentration was determined to be 7,101.4 pg/mL in serum, 4,286.1 pg/mL in plasma (citrate), 4,373.6 pg/mL in plasma (EDTA), and 6,311.1 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)

Interpolated concentrations of spike RGM-C in cell culture media.

The concentrations of RGM-C were measured in duplicates, interpolated from the RGM-C standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse RGM-C ELISA Kit (AB243685)

Example of mouse RGM-C standard curve in Sample Diluent NS.

The RGM-C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 16.3 pg/mL

검출 범위

46.88 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB239591

Anti-Hemojuvelin antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemojuvelin antibody [C1] (AB239591)

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Assay Kits

AB228554

Cell Counting Kit 8 (WST-8 / CCK8)

Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (AB228554)

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  • 4
Assay Kits

AB65473

WST-1 Assay Kit (Cell Proliferation)

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Assay Kits

AB197010

MTS Assay Kit (Cell Proliferation) (Colorimetric)

Biochemical assay - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse RGM-C ELISA Kit (ab243685) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RGM-C protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RGM-C with 16.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Serum", "range": "106 - 112 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "106 - 115 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "85 - 94 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "84 - 115 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "101 - 117 %", "average": "= 108" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243685-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB243685-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243685-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243685-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243685-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243685-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243685-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB243685-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse RGM-C Detector Antibody", "number":"AB243685-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse RGM-C Lyophilized Recombinant Protein", "number":"AB243685-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse RGM-C Capture Antibody", "number":"AB243685-CMP06", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hemojuvelin also known as HJV or RGMc is a glycosylphosphatidylinositol-anchored protein that plays a significant role in iron homeostasis. It has a molecular mass of about 42 kDa. Expression of hemojuvelin is mainly observed in the liver heart and skeletal muscles. This protein is essential for maintaining systemic iron levels and regulates how much iron is absorbed and stored in the body.
Biological function summary

Hemojuvelin interacts with other proteins to modulate iron metabolism. It acts as a coreceptor for bone morphogenetic proteins (BMPs) enhancing BMP signaling which is critical in regulating the peptide hormone hepcidin. Hemojuvelin itself is not part of a larger complex but it works closely with BMPs to control hepcidin expression. Hepcidin is the key hormone that dictates iron absorption and its release from macrophages maintaining iron balance.

Pathways

Hemojuvelin functions primarily within the BMP signaling and hepcidin regulatory pathways. The interaction with BMP6 is particularly critical. The BMP6-hemojuvelin complex boosts the SMAD signaling cascade resulting in increased hepcidin expression. This cascade impacts iron levels in the body by limiting intestinal iron absorption and controlling iron recycling by macrophages.

Hemojuvelin mutations can lead to juvenile hemochromatosis a condition characterized by excessive iron buildup in the body. This protein's dysfunction can severely affect iron homeostasis leading to detrimental health outcomes. Moreover its connection to the regulation of hepcidin links hemojuvelin to conditions like anemia of chronic disease where hepcidin expression is aberrantly increased or decreased. Understanding the interaction of hemojuvelin and hepcidin provides insights into the mechanisms behind these iron-related disorders.
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제품 프로토콜

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타겟 정보

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.
See full target information Hjv

대체 명칭 보기

Hfe2, Rgmc, Hjv, Hemojuvelin, Hemochromatosis type 2 protein homolog, Hemojuvelin BMP coreceptor, RGM domain family member C
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websiteProtocolBooklet
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Product promise

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