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AB309114

Mouse SOST ELISA Kit

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Mouse SOST ELISA Kit is a Sandwich ELISA for the measurement of Mouse SOST in Mouse in Cell Culture Media, Biofluids samples.

대체 명칭 보기

Sclerostin, Sost

3 이미지
Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Interpolated concentrations of mouse SOST in serum, plasma (citrate), and plasma (EDTA)

Interpolated concentration of native SOST was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Example of mouse SOST standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Standard curve comparison between mouse SOST SimpleStep ELISA kit (ab309114) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, EDTA Plasma, Citrate plasma, Serum

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.1 pg/mL

검출 범위

10.938 - 700 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse SOST SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOST protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse SOST with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "87 - 103 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "83 - 110 %", "average": "= 95" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309114-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309114-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309114-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309114-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309114-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309114-CMP06", "productcode":"" }, { "size":"1 x 6 Vial", "name":"Antibody Diluent CPI2", "number":"AB309114-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SOST Lyophilized Recombinant Protein", "number":"AB309114-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Capture Antibody", "number":"AB309114-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309114-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Detector Antibody", "number":"AB309114-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
See full target information Sost
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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