Mouse uPA ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- ELISA
Supplier Data
ELISA - Mouse uPA ELISA Kit (AB245727)
- sELISA
Supplier Data
Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
Interpolated concentrations of native uPA in mouse serum, plasma samples. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 7166 pg/mL in serum, 6222 pg/mL in plasma (citrate) and 7060 pg/mL in plasma (EDTA), and 4907 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
Interpolated concentrations of native uPA in mouse urine. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 272.6 ng/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
Interpolated concentrations of spiked uPA in cell culture media. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
Example of mouse uPA standard curve in Sample Diluent NS. The uPA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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How the assay works
Mouse uPA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse uPA ELISA Kit ab245727 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-uPA antibody [EPR22151-5] - BSA and Azide free (Capture) ab259584
- Anti-uPA antibody [EPR22151-66] - BSA and Azide free (Detector) ab259584
Precision
Recovery
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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